| 李广科,陈俊艳,付小娟.垃圾渗滤液暴露对拟南芥的毒效应研究[J].农业环境科学学报,2016,35(8):1458-1464. |
| 垃圾渗滤液暴露对拟南芥的毒效应研究 |
| Phytotoxicity of landfill leachate to Arabidopsis thaliana |
| 投稿时间：2016-01-25 |
| DOI：10.11654/jaes.2016-0119 |
| 中文关键词: 垃圾渗滤液 拟南芥 综合毒性 氧化损伤 |
| 英文关键词: landfill leachate Arabidopsis thaliana comprehensive toxicity oxidative damage |
| 基金项目:国家自然科学基金项目（21477070）；高等学校博士学科点专项科研基金项目（20121401110003） |
|
| 摘要点击次数: 2207 |
| 全文下载次数: 2025 |
| 中文摘要: |
| 为研究垃圾渗滤液的综合毒性，通过盆栽实验探讨了渗滤液对拟南芥早期、成熟期和后期生长发育的影响。结果表明：垃圾渗滤液会影响拟南芥整个生命期的生长情况，低浓度（0.1%）、短时间（24 h）的渗滤液暴露会促进拟南芥早期的萌发及根系生长，分别为对照的256%和324%，而高浓度（10%）、长时间（≥48 h）暴露则产生抑制作用，48 h的抑制效应分别为对照的67%和36%；在相同暴露时间下，芽长随暴露浓度变化不明显，仅在48 h呈现出差异，说明拟南芥幼苗的根系比芽更为敏感。不同浓度的渗滤液还会影响成熟期拟南芥的生长发育，诱导叶片氧化损伤并呈现浓度和时间双重依赖性，进而损伤其抗氧化防御系统。此外，高浓度（20%）渗滤液对晚期阶段拟南芥抽薹率和角果数的抑制效应较为明显（P<0.01），在暴露15 d后分别为对照的45%和43%。研究结果说明拟南芥可有效、简单、重复性地监测渗滤液的毒性，为渗滤液的植物监测提供理论依据。 |
| 英文摘要: |
| Landfill leachate can cause significant threats to the surrounding soil, surface water and groundwater. In the present research, the toxicity of landfill leachate to Arabidopsis thaliana was examined by detecting its growth and development during early, maturity and late stages. Our results showed that landfill leachate influenced the growth of Arabidopsis thaliana during the whole life cycle. Low concentrations of leachate(0.1%) and short time exposure(24 h) promoted the germination rates and root growth, which was 256% and 324% of the control, respectively; while high concentrations(10%) and long time exposure(≥48 h) showed inhibitory effects, with germination rates and root growth being 67% and 36% of control, respectively. However, shoot length showed a significant difference only under 48 h exposure, indicating that root length was more sensitive to leachate than shoot length. The landfill leachate also affected the growth of Arabidopsis thaliana during maturity stage, by inducing oxidative damages in concentration-effect and time-effect manners, and by further damaging the antioxidant defense system. In addition, high concentrations(20%) of leachate significantly inhibited the reproduction of Arabidopsis thaliana during late stage(P<0.01). The bolting rate and pod number decreased to 45% and 43% of control after 15-day leachate exposure, respectively. These results suggest that Arabidopsis thaliana is an efficient, simple and reproducible biomarker in monitoring the toxicity of landfill leachate. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
