| 张雪娜,贾海滨,李橙,王伟,张丽秀,冯圣东,杨志新.添加淀粉和通气对固定化Fusarium.sp修复煤矿区老化污染土壤HMW-PAHs的影响[J].农业环境科学学报,2016,35(9):1709-1716. |
| 添加淀粉和通气对固定化Fusarium.sp修复煤矿区老化污染土壤HMW-PAHs的影响 |
| Effect of starch and aeration addition on degradation of HMW-PAHs by immobilized Fusarium.sp in a polluted and aged soil of a coal mining area |
| 投稿时间：2016-04-28 |
| DOI：10.11654/jaes.2016-0596 |
| 中文关键词: 典型煤矿区 HMW-PAHs 固定化Fusarium.sp 淀粉 通气 土壤酶活性 |
| 英文关键词: a coal mining area HMW-PAHs immobilized Fusarium.sp starch aeration soil enzyme activity |
| 基金项目:河北省教育厅项目（Z2013058）；863专项（2012AA101403-3） |
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| 中文摘要: |
| 为了提高Fusarium.sp对PAHs的修复效果，在典型煤矿区老化污染土壤中添加淀粉和通气，经为期60 d的土壤培养试验，研究玉米秸秆固定化Fusarium.sp对土壤10种HMW-PAHs的修复效果及不同处理下土壤中酶活性变化规律。结果表明：HMW-PAHs（High molecular weight-PAHs）总量去除率表现为固定化菌剂+淀粉+通气处理（J+D+O，29.19%）≈固定化菌剂+淀粉处理（J+D，25.89%）>固定化菌剂处理（J，16.54%）；4环、5环和6环PAHs去除率在J+D+O和J+D两组处理间的差异均不显著，且与J处理相比均有显著提高（P<0.05）；三组处理对单个HMW-PAH的去除率分别为9.12%～21.73%、17.93%～43.12%、24.34%～35.79%，J+D+O和J+D处理对单个HMW-PAH的修复效果均有显著促进作用，其中，对BkF的去除增幅最大，分别增加了68.09%、63.78%。从酶活性规律看，土壤过氧化氢酶活性呈CK > J > J+D > J+D+O（P<0.05）的规律，且与10种PAHs单体的去除率呈显著或极显著负相关；土壤木质素过氧化物酶活性却与Chry、BkF、InP、DbA、BghiP去除率呈显著正相关。综上认为，不同处理对单个PAHs的去除具有其选择性，且添加淀粉的两处理均显著提高了土壤中HMW-PAHs的修复效果。 |
| 英文摘要: |
| The soil incubation experiment was performed to study the effect of starch and aeration addition on HMW-PAHs degradation by Fusarium.sp in polluted and aged soil of a coal mining area. After 60 days incubation, the degradation rate of 10 HMW-PAHs as well as the change rule of soil enzyme activities were analyzed. The results showed that the treatments were in the following order in terms of ∑10 HMW-PAHs degradation rate:immobilized Fusarium.sp+starch+aeration(J+D+O, 29.19%)≈immobilized Fusarium.sp+starch(J+D, 25.89%)>immobilized Fusarium.sp(J, 16.54%). There was no significant difference between J+D+O and J+D for the degradation of ∑4-rings, ∑5-ring and ∑6-ring PAHs, but their degradation rates were higher than J significantly(P<0.05). The degradation range of 10 individual HMW-PAHs were 9.12%~21.73%, 17.93%~43.12%, 24.34%~35.79% in J, J+D and J+D+O treatments respectively. The results demonstrated that far higher degradation rates of the 10 individual HMW PAHs were achieved in J+D+O and J+D treatments than those in J treatment, but there was no significant difference between J+D+O and J+D. The removal rates of BkF presented the highest increase extent in the two treatments and increased 68.09%, 63.78% respectively. In addition, the treatments were in the following order in terms of catalase enzyme activities:CK > J > J+D > J+D+O(P<0.05). We found that there were significant(P<0.05) linear negative correlation between the removal rates of the 10 individual HMW-PAHs and catalase activity in soil. However, the degradation rate of Chry, BkF, InP, DbA, BghiP had significant(P<0.05) linear positive correlation with lignin peroxidase activity. In conclusion, the individual PAH was removed by different treatments selectively, and the degradation rate were significantly improved by adding starch. |
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