文章摘要
江群,杨帆,朱墨染,赵璐璐,闫立龙,张颖.玉米秸秆生物炭固定化Acinetobacter lwoffii DNS32性能研究[J].农业环境科学学报,2017,36(2):382-386.
玉米秸秆生物炭固定化Acinetobacter lwoffii DNS32性能研究
The degradation properties of immobilized bacteria using corn stover-derived biochars as carriers
投稿时间:2016-08-09  
DOI:10.11654/jaes.2016-1018
中文关键词: 生物炭  固定化菌剂  阿特拉津  生物降解
英文关键词: biochar  immobilized bacteria  atrazine  bio-degradation
基金项目:国家自然科学基金青年科学基金项目(316000413);中国博士后科学基金会项目(2015M581417);黑龙江省博士后基金项目(LBH-Z15012)
作者单位E-mail
江群 东北农业大学资源与环境学院, 哈尔滨 150030  
杨帆 东北农业大学资源与环境学院, 哈尔滨 150030  
朱墨染 东北农业大学资源与环境学院, 哈尔滨 150030  
赵璐璐 东北农业大学资源与环境学院, 哈尔滨 150030  
闫立龙 东北农业大学资源与环境学院, 哈尔滨 150030  
张颖 东北农业大学资源与环境学院, 哈尔滨 150030 zhangying_neau@163.com 
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中文摘要:
      用玉米秸秆制备生物炭,并以其作为固定化菌剂的廉价载体,与阿特拉津降解菌Acinetobacter lwoffii DNS32制备成具有吸附-降解性能的新型菌剂,用以降解水溶液中阿特拉津。结果表明:固定化菌剂可在40 h内将100 mg·L-1的阿特拉津降解94%,降解率比游离菌高24%;固定化菌剂在pH=5和pH=10时,降解率分别为42%和35%,说明其具有更好的pH适应性;温度为10℃时,固定化菌剂的降解率比游离菌高14%,说明其具有更好的耐寒性。为期30 d的模拟修复阿特拉津污染的实验表明:生物炭固定化菌剂在30 d后仍然具有活性,该固定化菌剂具有高效且持久的阿特拉津污染修复效果。
英文摘要:
      The biodegradation of atrazine in aqueous solution by immobilized bacteria using corn stover-derived biochars were studied. The degradation bacteria screened by cooperators in our laboratory was named as Acinetobacter lwoffii DNS32. Those strains grew well on corn stover-derived biochars, and the degradation rate of immobilized bacteria could reach 94% at 40 h, which implied that their degradation efficiency was higher than that of free bacteria(70%). At pH=5 and pH=10, the degradation rate of immobilized bacteria was 42% and 35% respectively, which showed they had better adaptability to pH. At 10℃, the degradation rate of immobilized bacteria was 14% higher than that of free bacteria, which certified that they had better cold tolerance. In the simulation experiments treating atrazine pollution water, immobilized degradation bacteria were still active after 30 d, indicated that immobilized bacteria had efficient and lasting repairing effect for atrazine contaminated water.
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