| 刘迎,姜蕾,潘波,林勇.斑马鱼胚胎经丙草胺暴露后对其仔鱼致畸效应的研究[J].农业环境科学学报,2017,36(3):481-486. |
| 斑马鱼胚胎经丙草胺暴露后对其仔鱼致畸效应的研究 |
| Teratogenic effects of embryonic exposure to pretilachlor on the larvae of zebrafish |
| 投稿时间：2016-11-08 |
| DOI：10.11654/jaes.2016-1418 |
| 中文关键词: 丙草胺 斑马鱼 致畸效应 Tbx2 BMP-2 BMP-4 shha ihha |
| 英文关键词: pretilachlor zebrafish teratogenic effect Tbx2 BMP-2 BMP-4 shha ihha |
| 基金项目:海南省自然科学基金项目（20153125）；海南省社会发展科技专项（2015SF35） |
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| 中文摘要: |
| 为探讨斑马鱼胚胎暴露于丙草胺后对斑马鱼仔鱼的致畸效应和致畸机理，研究了斑马鱼胚胎暴露于0.50、1.00、1.50 mg·L-1丙草胺染毒液120 h后仔鱼的畸形表型及比率，并通过检测与畸形器官发育相关的基因表达探讨致畸机理。结果发现，丙草胺暴露后诱导斑马鱼仔鱼出现心包囊肿、躯干弯曲、游囊关闭等畸形表型，随着暴露浓度的增加，各畸形症状比例增大。1.00、1.50 mg·L-1暴露组仔鱼心包囊肿率分别为15.56%（P<0.05）、25.56%（P<0.01），脊柱弯曲率分别为27.78%（P<0.01）、35.56%（P<0.01）；0.50、1.00、1.50 mg·L-1暴露组仔鱼游囊关闭率分别为20%（P<0.05）、37.78%（P<0.01）和60%（P<0.01）。丙草胺显著降低了心脏发育相关基因Tbx2[1.00 mg·L-1（P<0.01）、1.50 mg·L-1（P<0.05）]，骨骼发育相关基因BMP-2[1.50 mg·L-1（P<0.05）]、BMP-4[1.00 mg·L-1（P<0.01）、1.50 mg·L-1（P<0.01）]，游囊发育相关基因shha[1.50 mg·L-1（P<0.05）]、ihha[1.00 mg·L-1（P<0.05）、1.50 mg·L-1（P<0.05）]的表达水平。研究表明，丙草胺通过影响心脏、骨骼和游囊相关基因的表达引起斑马鱼仔鱼的以上器官发育异常。 |
| 英文摘要: |
| To investigate the teratogenic effect and mechanism of abnormality of embryonic exposure to pretilachlor on the larvae of zebrafish, the malformation phenotype and abnormality rate of the larvae were studied after embryos were esposed to 0.50, 1.00 and 1.50 mg·L-1 pretilachlor from 6 h post fertilization(hpf) to 120 hpf, and also the abnormality mechanism was investigated by testing the gene expression related organ development. The results showed that pretilachlor could induce malformations of larvae, including pericardial cyst,spinal curvature and uninflated swim bladder, and the percentage of three kinds of abnormalities increased with the increase of exposure dose. The pericardial cyst rate were 15.56%(P < 0.05), 25.56%(P < 0.01) and spinal curvature rate were 27.78%(P < 0.01), 35.56%(P < 0.01) respectively after embryo exposed to 1.00, 1.50 mg·L-1 pretilachlor. The uninflated swim bladder rate were 20%(P < 0.05), 37.78%(P < 0.01), 60%(P < 0.01) respectively after embryo exposed to 0.50, 1.00, 1.50 mg·L-1 pretilachlor. mRNA expressions of heart development related gene Tbx2[1.00 mg·L-1(P < 0.01), 1.50 mg·L-1(P < 0.05)], skeleton development related genes BMP-2[1.50 mg·L-1(P < 0.05)], BMP-4[1.00 mg·L-1(P < 0.01), 1.50 mg·L-1(P < 0.01)] and swim bladder development related genes shha[1.50 mg·L-1(P < 0.05)], ihha[1.00 mg·L-1(P < 0.05), 1.50 mg·L-1(P < 0.05)] were reduced significantly after embryos were esposed to pretilachlor. The results deduced that pretilachlor could induce heart, skeleton and swim bladder developmental anomaly by supressing the mRNA expressions of above-mentioned oragan development related gene. |
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