文章摘要
陈红苗,许鹏,吴中强,井维鑫,王兰.NAC对河南华溪蟹卵巢镉氧化损伤的缓解作用[J].农业环境科学学报,2017,36(6):1087-1092.
NAC对河南华溪蟹卵巢镉氧化损伤的缓解作用
Alleviation of cadmium-induced oxidative stress in Sinopotamon henanense ovarian tissue by N-acetylcysteine
投稿时间:2016-12-02  
DOI:10.11654/jaes.2016-1545
中文关键词: 河南华溪蟹  卵巢  N-乙酰半胱氨酸(NAC)  氧化损伤  
英文关键词: Sinopotamon henanense  ovary  N-acetylcysteine(NAC)  oxidative damage  cadmium
基金项目:国家自然科学基金项目(31672293);高等学校博导类基金项目(20111401110010);山西省特色学科重点项目(2011-SXDX-SWX-003);山西省高等学校科技创新项目(113548901027)
作者单位E-mail
陈红苗 山西大学生命科学学院, 太原 030006  
许鹏 山西大学生命科学学院, 太原 030006  
吴中强 山西大学生命科学学院, 太原 030006  
井维鑫 山西大学生命科学学院, 太原 030006  
王兰 山西大学生命科学学院, 太原 030006 lanwang@sxu.edu.cn 
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中文摘要:
      采用慢性镉暴露同时加入不同浓度N-乙酰半胱氨酸(NAC)的方法,以河南华溪蟹卵巢组织镉含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)活性,脂质过氧化水平(MDA含量)和卵巢组织细胞结构的变化为指标,探究了NAC对河南华溪蟹(Sinopotamon henanense)卵巢镉氧化损伤的缓解作用。实验设置对照组、镉(0.725 mg·L-1)单一作用组、镉(0.725 mg·L-1)和NAC(0.1、0.2、0.4 g·L-1)联合作用组,结果显示:镉单一作用下,卵巢组织镉含量和MDA含量显着高于对照组;SOD、CAT和GPx活性在不同时间点显着升高;组织显微结构出现不同程度损伤。NAC和镉联合作用下,卵巢组织镉含量在14 d和28 d显着降低;SOD活性于14 d显着降低;CAT、GPx活性和MDA含量均在28 d降低,并逐渐恢复到正常水平;随着时间的延长和NAC浓度的增加,卵巢组织细胞损伤程度出现改善,核膜逐渐完整,仅有少量染色质凝集,细胞内及细胞核内空泡化现象基本消失,卵巢组织结构趋于正常化。实验结果表明,NAC对镉诱导的河南华溪蟹的生殖毒性具有缓解作用,同时进一步证实了氧化损伤是镉生殖毒性的重要机制之一。
英文摘要:
      The aim of this study was to investigate the effects of N-acetylcysteine(NAC) on alleviation of cadmium-induced oxidative stress in Sinopotamon henanense ovarian tissue using the following six indicators:the tissue accumulation of Cd; the activities of antioxidant enzymes superoxide dismutase(SOD), catalase(CAT), and glutathione peroxidase(GPx); the content of malonyldialdehyde(MDA); and the changes in the microstructure of ovarian tissue. The experiment included three groups:the control group, the Cd2+(0.725 mg·L-1) group, and the group of Cd2+(0.725 mg·L-1) combined with NAC at three different levels of concentration(0.1, 0.2, or 0.4 g·L-1). The results showed that under the action of cadmium alone, with increasing Cd2+ concentration the accumulation of Cd and the content of MDA significantly increased as compared to that in the control group. The activities of SOD, CAT, and GPx increased at different time-points. The microstructure of ovarian tissue was damaged at different levels. For the group of Cd2+ combined with NAC at three different levels of concentration, the content of Cd in ovarian tissue decreased significantly on the 14th and the 28th days. The activity of SOD decreased significantly on the 14th day. The activities of CAT, GPx, and the content of MDA had a significant decrease on the 28th day, and returned to normal levels gradually. With the extension of time and the increase in NAC concentration, the damage to ovarian tissues was alleviated. That is, nuclear membranes were gradually repaired, and there was only a small quantity of chromatin agglutinate. The vacuolation in cells and nuclei almost disappeared, with the structure of ovarian cells returning to normal conditions. The results indicated that NAC could alleviate the cadmium-induced reproductive toxicity in Sinopotamon henanense. The results of our study support the argument that oxidative stress is one of the important underlying mechanisms of reproductive toxicity of cadmium.
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