| 魏琳琳,杨殿林,侯萌瑶,倪土,李刚,修伟明,王慧,赵建宁.氮高效转基因水稻OsNRT2.3b对土壤氨氧化细菌群落多样性的影响[J].农业环境科学学报,2017,36(6):1149-1159. |
| 氮高效转基因水稻OsNRT2.3b对土壤氨氧化细菌群落多样性的影响 |
| Effect of N-efficient transgenic rice on diversity and community structure of soil ammonia-oxidizing bacteria |
| 投稿时间：2017-01-20 |
| DOI：10.11654/jaes.2017-0116 |
| 中文关键词: 氮高效转基因水稻 氨氧化细菌 多样性 PCR-DGGE |
| 英文关键词: N-efficient transgenic rice ammonia-oxidizing bacteria diversity PCR-DGGE |
| 基金项目:国家自然科学基金项目（31301855）；转基因生物新品种培育重大专项（2016ZX08012005） |
| 作者 | 单位 | E-mail | | 魏琳琳 | 农业部环境保护科研监测所, 农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点开放实验室, 天津 300191 | | | 杨殿林 | 农业部环境保护科研监测所, 农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点开放实验室, 天津 300191 | | | 侯萌瑶 | 农业部环境保护科研监测所, 农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点开放实验室, 天津 300191
东北农业大学资源与环境学院, 哈尔滨 150030 | | | 倪土 | 农业部环境保护科研监测所, 农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点开放实验室, 天津 300191 | | | 李刚 | 农业部环境保护科研监测所, 农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点开放实验室, 天津 300191 | | | 修伟明 | 农业部环境保护科研监测所, 农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点开放实验室, 天津 300191 | | | 王慧 | 农业部环境保护科研监测所, 农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点开放实验室, 天津 300191 | | | 赵建宁 | 农业部环境保护科研监测所, 农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点开放实验室, 天津 300191 | zhaojn2008@163.com |
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| 中文摘要: |
| 以氮高效转基因水稻OsNRT 2.3b两个不同株系N-04和N-08为研究对象，以非转基因亲本日本晴（Nipp）为对照，在田间小区试验条件下，设施氮和不施氮两种处理，采用变性梯度凝胶电泳（PCR-DGGE）技术，分析了氮高效转基因水稻在生长期对土壤氨氧化细菌群落多样性的影响。研究发现，水稻土壤氨氧化细菌丰富度指数在各生长期内品种间均不存在显着差异。两种处理条件下N-04的土壤氨氧化细菌香农-威纳指数仅在拔节期与Nipp有显着差异，其余生育期均无显着差异；在施氮条件下N-08的土壤氨氧化细菌香农-威纳指数在拔节期和抽穗扬花期与Nipp存在显着差异，不施氮条件下仅拔节期出现显着差异。两种处理条件下，N-04 的土壤氨氧化细菌均匀度指数与Nipp相比整个生长期均无显着差异，而N-08在拔节期显着低于Nipp.测序结果表明，施氮和不施氮处理下氮高效转基因水稻（N-08和N-04）与Nipp相比土壤中拥有更多的亚硝化螺旋菌属（Nitrosospira）和亚硝化单胞菌属（Nitrosomonas）.研究表明，氮高效转基因水稻在个别生育期对香农-威纳指数和均匀度指数有显着差异，且其更有利于促进土壤铵态氮向硝态氮的转化。 |
| 英文摘要: |
| Our study investigated the effect of N-efficient transgenic rice of different strains(N-04 and N-08) and its parent on the diversity and community structure of soil ammonia-oxidizing bacteria. Regarding the field plot experiment, we analyzed the effect of the N-efficient transgenic rice on diversity and community structure of soil ammonia-oxidizing bacteria with nitrogenous and non-nitrogenous fertilizer application via a denaturing gradient gel electrophoresis(DGGE) technique. No significant difference in richness index(S) between the N-efficient transgenic rice and Nipp was found. Excluding the elongation stage, N-04 and Nipp had no significant difference in Shannon-Wiener index(H) between nitrogenous and non-nitrogenous fertilizer application. During the elongation, heading, and flowering stages with nitrogenous fertilizer application and the elongation stage without nitrogenous fertilizer application, N-08 had significant effects on the H. Under the conditions of nitrogenous and non-nitrogenous fertilizer application, N-04 had no significant difference on the evenness index En) during all growing stages, and N-08 only had significant difference with regard to Nipp during the elongation stage. The sequence and phylogenetic analysis indicated that N-04 and N-08 had more bands than Nipp, and these bands belonged to Nitrosospira and Nitrosomonas. Therefore, N-efficient transgenic rice had significant effects on the H and En of soil ammonia-oxidizing bacteria during certain rice growing stages, and it was more effective than its parent was for accelerating NH4+ transfer to NO3-. |
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