| 宋立超,张薇,钮旭光,李瑞,曹阳,赵浩宇,周立炀.焦化厂区地肤根际芘降解细菌筛选和促生潜力研究[J].农业环境科学学报,2017,36(11):2275-2280. |
| 焦化厂区地肤根际芘降解细菌筛选和促生潜力研究 |
| Screening of pyrene-degrading bacteria from Kochia scoparia rhizosphere in coking plant and study of plant growth-promoting ability |
| 投稿时间：2017-07-17 |
| DOI：10.11654/jaes.2017-0997 |
| 中文关键词: 根际促生菌 地肤 多环芳烃 降解 |
| 英文关键词: plant growth-promoting rhizobacteria Kochia scoparia polycyclic aromatic hydrocarbons degradation |
| 基金项目:国家自然科学基金项目（41703103，31401322）；农业部东北耕地保育重点实验室项目（2015NYBKFT-4） |
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| 中文摘要: |
| 为强化焦化厂土壤多环芳烃原位植物-微生物修复的应用，提供具有降解多环芳烃功能的植物促生菌，分别以芘和1-氨基环丙烷-1-脱氨酶（ACC脱氨酶）为唯一碳源和氮源，采用富集培养法对某焦化厂优势植物地肤根际土壤中的功能菌株进行分离。研究分离的菌株对芘的降解能力和对植物的促生特性；通过种子萌发试验，以芘为碳源，研究菌株对地肤种子发芽率和根长的影响。多次富集后，得到7株菌，经鉴定命名为考克氏菌KSB1、芽孢杆菌KSB2、类香味菌KSB3、沙雷菌KSB4、副球菌KSB5、松鼠葡萄球菌KSB6、芽孢杆菌KSB7。经过14 d的降解实验，菌株KSB1、KSB2、KSB4、KSB5和KSB7均可降解约58%以上的芘。菌株KSB2、KSB4和KSB7的ACC脱氨酶活性大于3.5 M α-KB·mg-1·h-1。在种子萌发实验中，菌株KSB2、KSB4和KSB7均可显著提高芘胁迫下（芘浓度10~25 mg·L-1）地肤种子的发芽率和芽长，其中KSB7的效果最好，与对照相比对地肤发芽率和芽长分别提高了56.76%和88.9%，表明菌株KSB7在焦化厂污染土壤的地肤-微生物联合修复中具有较大的应用潜力。 |
| 英文摘要: |
| In order to enhance in situ bioremediation of coking plant soils contaminated by polycyclic aromatic hydrocarbons(PAHs) and obtain plant growth-promoting bacteria that can degrade PAHs, functional strains in the rhizosphere of Kochia scoparia, which is a typical plant in coking plants, were obtained using the enrichment culture method. Pyrene and 1-amino cyclopropane-1-deaminase(ACC) were used as the sole carbon and nitrogen sources, respectively. The pyrene degradation ability and plant growth-promoting characteristics of the isolated strains were studied. The effect of the strains on the germination rate and root length of Kochia scoparia seeds were also analyzed using pyrene as the carbon source. After several enrichment times, 7 strains were identified and named as Kocuria sp. KSB1, Bacillus sp.KSB2, Myroides sp. KSB3, Serratia. sp. KSB4, Paracoccus sp. KSB5, Staphylococcus sciuri. KSB6, and Bacillus sp.KSB7. In the degradation experiment, more than 58% of pyrene could be degraded in 14 days by strains KSB1, KSB2, KSB4, KSB5, and KSB7. The activity of ACC deaminase was more than 3.5 M α-KB mg-1·h-1 in KSB2, KSB4, and KSB7. Strains KSB2, KSB4, and KSB7 significantly improved the germination rate and root length under pyrene stresses of 10~25 mg·L-1. Compared to CK, KSB7 increased the germination rate by 56.76% and shoot length by 88.9%, respectively. The results show that strain KSB7 has great potential in the bioremediation of contaminated soils of coking plants using Kochia scoparia. |
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