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| 入侵植物黄顶菊不同器官和不同发育阶段DNA表观遗传多样性变化特征 |
| Epigenetic diversity variation characteristics of Flaveria bidentis genome DNA of different organs and in different developmental stages |
| 投稿时间：2018-09-17 修订日期：2018-11-05 |
| DOI： |
| 中文关键词: 黄顶菊 DNA甲基化 MSAP 表观遗传学 |
| 英文关键词: Flaveria bidentis DNA methylation MSAP epigenetics |
| 基金项目:国家自然基金青年科学(31401811)；天津市应用基础与前沿技术研究计划(青年项目,15JCQNJC15300) |
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| 中文摘要: |
| DNA甲基化是植物DNA普遍存在的一种表观遗传修饰方式,不仅在植物快速适应新环境中有着重要的作用,还参与植物生长发育和器官分化特异性过程。本研究通过构建优化的MSAP体系来分析入侵植物黄顶菊不同器官和同一器官不同发育阶段组织的甲基化变异特征,结果表明：器官特异性MSAP体系利用筛选的13对引物共扩增536条条带,其中引物EhHM7对表观遗传多样性贡献率最大,多态性百分比为92.45%；发育阶段特异性MSAP体系利用筛选的14对引物共扩增407条条带,其中EcHM1对表观遗传多样性贡献率最大,多态性百分比为80.56%。甲基化模式分析结果显示,黄顶菊不同器官(根、茎和叶)间以及相同器官不同发育阶段(老叶和嫩叶)组织间均表现出显著的甲基化水平差异性,其中,茎组织的各甲基化发生率最高,半甲基化、全甲基化和整体甲基化率分别达到30.28%、19.37%和49.66%,老叶组织的全甲基化和整体甲基化率显著高于嫩叶组织,分别达到33.29%和52.77%。主成分分析结果显示,黄顶菊叶片个体分布较根、茎器官的个体分布更为密集,且嫩叶较老叶密集,表明根、茎器官间存在较大的个体差异,这种个体差异大于黄顶菊叶片的个体间差异,且老叶的个体差异大于嫩叶,这种个体间差异在样品采集时不可忽视。所以,在利用表观遗传学方法进行黄顶菊入侵性的研究中,必须要制定科学的采样方案,把植物器官和生长发育阶段特异性作为一个重要因素考虑在内。 |
| 英文摘要: |
| As one of the epigenetic modifications, DNA methylation has become ubiquitous in plant genome DNA. It not only plays an important role in rapid adaptation to new environments, but also participates in process of plant growth and organ differentiation. In this study, we constructed an optimized MSAP system to analyze the methylation variation characteristics of different organs and different developmental stages of the same organs of Flaveria bidentis. The results showed that the organ-specific MSAP system amplified 536 bands by 13 pairs of primers, in which the primer EhHM7 made greatest contribution to epigenetic diversity and the percentage of polymorphism was 92.45%. The developmental stage-specific MSAP system amplified 407 bands by 14 pairs of primers, in which EcHM1 had the most contribution rate to epigenetic diversity and the percentage of polymorphism was 80.56%. The results of Methylation pattern analysis showed that there were significant differences in methylation levels among different organs (roots, stems and leaves) and different developmental stages of the same organs (old leaves and young leaves), among which the methylation rate of stem tissues was the highest, and the rates of semi-methylated, full-methylated and overall methylated reached 30.28%, 19.37% and 49.66%, respectively. Full-methylated and overall methylated rate of old leaf tissue were significantly higher than that of the young leaf tissue, reaching 33.29% and 52.77% respectively. Principal component analysis showed that the individual distribution of leaves of Flaveria bidentis was more intensive than that of roots and stem organs, and the young leaves were denser than the old leaves, which indicating that there were larger individual differences between roots and stems. This individual difference was greater than that of leaves of Flaveria bidentis and the individual difference of old leaves is greater than that of young leaves, which cannot be ignored in sample collection. Therefore, during the studies on the invasiveness of Flaveria bidentis by using epigenetic methods, it is necessary to formulate a scientific sampling plan and take plant organ and growth stage specificity as an important factor. |
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