文章摘要
里氏木霉利用玉米秸秆形成类胡敏酸(HAL)的特异性研究
Specificity of the humic acid-like(HAL) by using Trichoderma reesei inoculated corn straw
投稿时间:2019-04-02  修订日期:2019-05-01
DOI:
中文关键词: 腐殖化  玉米秸秆  里氏木霉  腐殖物质的特异性  类胡敏酸
英文关键词: Humification  Corn straw  Trichoderma reesei  Specificity of humic substances  Humic acid-like
基金项目:国家重点研发计划项目(2016YFD0200304); 国家自然科学基金项目(41571231); 吉林省秸秆综合利用平台项目(2014C -1)
作者单位E-mail
张一枫 吉林农业大学 495575720@qq.com 
窦森 吉林农业大学 dousen1959@126.com 
叶淑芬 吉林农业大学  
张丹丹 吉林农业大学  
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中文摘要:
      【目的】为探明里氏木霉(Trichoderma reesei, T. reesei)在腐殖化过程中,不同培养时间下利用玉米秸秆得到的培养产物(HLS)的碱提取物——类胡敏酸(HAL)的结构特征是否具有与土壤胡敏酸相似的特异性。【方法】通过固体培养的方式模拟秸秆堆肥,在玉米秸秆中添加里氏木霉进行为期30天的腐殖化培养,并以灭菌后不添加微生物的处理作为对照,在培养的0d、15d、30d采样观察研究。通过元素组成、红外光谱及差热的技术手段对里氏木霉利用玉米秸秆形成的类胡敏酸(HAL)进行分析,与土壤的胡敏酸(HA)比较观察其特异性。【结果】对比CK,在模拟腐殖化的过程中,接种里氏木霉可以显著改变其HAL的结构特征,其芳香性/脂族性的比例变化符合多酚学说。里氏木霉可以在30d内将HAL的元素结构集中在O/C=0.528、H/C=1.322左右,与土壤HA的O/C比值、H/C比值差异缩减了71.08%、49.25%,使其更加接近土壤HA的结构特征。【结论】总体来看,里氏木霉在分解矿化玉米秸秆中纤维素和木质素的同时,可以有效促进玉米秸秆向腐殖质的转化,其形成的HAL的结构变化支持了多酚学说。里氏木霉培养下的HAL可随着腐殖化培养时间的增加,与土壤HA的结构特征差异持续缩小,逐渐具有特异性。里氏木霉在降低HAL脂族性、增强芳构化程度和热稳定性方面的能力很强,可以高效促进玉米秸秆的腐殖化进程。
英文摘要:
      [Objective]In order to investigate whether the structural features of Humic acid-like (HAL)—the alkaline extract of culture product (Humic-like substances, HLS) obtained from corn straw during the humification process of Trichoderma reesei (T. reesei)have similar specificities to that of HA in soil. [Method] Corn straw composting is simulated by solid culture. The humus process of corn straw is simulated by two treatments (adding T. reesei and uninoculated control after sterilization(CK) ) for a period of 30 days, and the samples are collected at 0d, 15d and 30d for observation. The HAL formed by T. reesei using corn straw were analyzed by elemental composition, infrared spectroscopy and differential thermal technique, and the specificity of which is observed by comparison with that of HA in soil. [Results] Compared with CK, inoculation of T. reesei can significantly change the structural characteristics of HAL and the changes of the ratio of aromatic/aliphatic in the process of simulating humification in it is consistent with the polyphenol theory. T. reesei can concentrate the elemental structure of HAL at O/C=0.528 and H/C=1.322 at 30 days, and the difference between HAL and HA is reduced by 71.08% and 49.25%, closer to the structural characteristics of HA. [Conclusion] In general, T. reesei can effectively promote the conversion of corn straw to humus while decomposing cellulose and lignin in mineralized corn straw, and the structural change of HAL formed by it supports the polyphenol theory. With the increase of humus culture time, the difference between the structure characteristics of HAL in T. reesei culture and that of HA in soil continues to decrease and gradually becomes specific. T. reesei is highly capable of reducing HAL aliphaticity, enhancing aromatization and thermal stability, and can efficiently promote the humification process of corn straw.
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