文章摘要
狄军贞,李拓达,赵微.甘蔗渣碳源释放规律及其硫酸盐还原菌利用性试验[J].农业环境科学学报,2019,38(5):1151-1157.
甘蔗渣碳源释放规律及其硫酸盐还原菌利用性试验
Release law of sugarcane slag carbon source and orthogonal testing of sulfate-reducing bacteria
投稿时间:2018-08-02  
DOI:10.11654/jaes.2018-0994
中文关键词: 甘蔗渣  硫酸盐还原菌  还原糖  碳源
英文关键词: bagasse  sulfate-reducing bacteria  reducing sugar  carbon source
基金项目:国家自然科学基金项目(41672247,41102157);辽宁省"兴辽英才计划"项目(XLYC1807159);辽宁省教育厅科研基金(LJYL031)
作者单位
狄军贞 辽宁工程技术大学土木工程学院, 辽宁 阜新 123000 
李拓达 辽宁工程技术大学土木工程学院, 辽宁 阜新 123000 
赵微 辽宁工程技术大学土木工程学院, 辽宁 阜新 123000 
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中文摘要:
      针对传统有机碳源使用费用高、易产生二次污染等问题,采用农业废弃物甘蔗渣作为碳源,开展甘蔗渣在不同pH、不同粒径、不同投加量条件下的单因素试验和在不同硫酸盐还原菌(SRB)投加量、不同甘蔗渣粒径及其不同投加量条件下的正交试验,研究碳源释放规律以及SRB利用甘蔗渣的最佳组合条件。结果表明,在pH值为7的体系、甘蔗渣粒径和投量分别为60目、3.5 g·100 mL-1废水时,甘蔗渣中纤维素水解速率及葡萄糖水解速率均较慢,有利于还原糖的持续积累。在100 mL废水中,SRB投加量为71.2 mg、甘蔗渣粒径和投量分别为100目和4.5 g时,SO42-去除效果最好、还原糖水解速率最小,为正交试验的最佳组合。在此组合条件下,甘蔗渣持续供给碳源96 h后,反应体系中SO42-去除率和还原糖释放量仍分别高达75.23%、64.03 mg·g-1,氧化还原电位(ORP)低至-224 mV。
英文摘要:
      Aiming at the problems of high cost and easy release of secondary pollution during the use of traditional organic carbon sources, single factor experiments and orthogonal experiments were performed to study the release law of the carbon source and the optimal condition of sulfate-reducing bacteria (SRB) using bagasse. The single factor experiments were carried out at different pH values, with different bagasse particle sizes, and with different bagasse dosages. The orthogonal experiments were carried out with different SRB dosages, different bagasse particle sizes, and different bagasse dosages. The results of the single factor experiments showed that the hydrolysis rates of cellulose and glucose in bagasse were slower when the wastewater pH value was 7 and the particle size and dosage of bagasse were 60 mesh and 3.5 g·100 mL-1, respectively, which would be beneficial to the continuous accumulation of reducing sugar. The results of the orthogonal experiments showed that the removal effect of SO42- was the best and the hydrolysis rate of reducing sugar was the lowest when the SRB dosage was 71.2 mg·100 mL-1 and the particle size and dosage of bagasse were 100 mesh and 4.5 g·100 mL-1, respectively, which was determined as the best combination of orthogonal experimental conditions. Under the best combination conditions, the removal rate of SO42- and release amount of reducing sugar in the solution were still 75.23% and 64.03 mg·g-1, respectively, and the oxidation-reduction potential (ORP) was -224 mV, after the bagasse was continuously supplied as a carbon source for 96 hours.
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