文章摘要
刘佳茜,侯丽君,刘婷婷,王沛媛,高祥,林雁冰.PBAT地膜降解菌的筛选及其降解特性研究[J].农业环境科学学报,2021,40(1):129-136.
PBAT地膜降解菌的筛选及其降解特性研究
Isolation of PBAT plastic-degrading bacteria and their degradation characteristics
投稿时间:2020-07-29  
DOI:10.11654/jaes.2020-0888
中文关键词: 聚己二酸/对苯二甲酸丁二酯  微生物降解  农膜  塑料膜片
英文关键词: polyadipate/butylene terephthalate  microbiological degradation  agricultural film  plastic mulch
基金项目:陕西省重点研发计划项目(2019NY-187);杨凌示范区科技计划项目(2018SF-01)
作者单位E-mail
刘佳茜 西北农林科技大学生命科学学院, 陕西 杨凌 712100  
侯丽君 西北农林科技大学生命科学学院, 陕西 杨凌 712100  
刘婷婷 西北农林科技大学理学院, 陕西 杨凌 712100  
王沛媛 西北农林科技大学生命科学学院, 陕西 杨凌 712100  
高祥 西北农林科技大学创新实验学院, 陕西 杨凌 712100  
林雁冰 西北农林科技大学生命科学学院, 陕西 杨凌 712100 linyb2004@nwafu.edu.cn 
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中文摘要:
      为了得到能够高效降解聚己二酸/对苯二甲酸丁二酯(PBAT)地膜的菌种资源,以PBAT塑料地膜为主要研究对象,从长期存在塑料的环境中分离筛选塑料降解菌,共得到6株潜在PBAT塑料降解菌,通过对PBAT降解性能进行测定,筛选出两株高效的PBAT降解菌株Pseudomonas sp.RD1-3和Pseudomonas sp.N1-2。在28℃条件下降解8周后,菌株RD1-3和N1-2对PBAT的降解率分别为6.88%±0.06%和6.49%±0.01%。两株菌能使PBAT塑料膜片的表面粗糙程度增加并出现大量沟壑、坑洞及褶皱。此外,两菌株降低了PBAT塑料膜片表面的疏水性,同时向膜片稳定的化学结构中引入—C≡N、—C=C—以及—OH等新的极性官能团。研究初步认定菌株RD1-3以及N1-2对PBAT均具有较好的降解能力。
英文摘要:
      PBAT(polyadipate/butylene terephthalate)mulch is one of the most common biodegradable mulches. It is eco-friendly and has good heat resistance and ductility. PBAT mulch has gradually been commercialized, and the output of it is currently increasing year by year. We obtained bacterial resources that could efficiently degrade PBAT mulch film, by isolating plastic-degrading bacteria from environments where plastics have existed for a long time. A total of 6 potential PBAT plastic-degrading bacterial strains were isolated, and two highly efficient PBAT-degrading strains(Pseudomonas sp. RD1-3 and Pseudomonas sp. N1-2)were identified based on their PBAT degradation performance. After 8 weeks of degradation at 28℃, strains RD1-3 and N1-2 showed PBAT degradation rates of 6.88%±0.06% and 6.49%±0.01%, respectively. The two strains increased the surface roughness of the PBAT mulch and formed many gullies, pits, and folds on the surface. In addition, the two strains reduced the hydrophobicity of the PBAT plastic mulch surface, and introduced new polar functional groups such as -C≡N, -C=C- and -OH into the mulch. The strains RD1-3 and N1-2 are preliminarily determined to have good PBAT degradation ability. The results of this study expand the resource library of PBAT plastic degradation bacteria and provide theoretical support for further research into PBAT plastic degradation.
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