| 牟凤利,杨京民,刘翠,刘才鑫,刘梅,陈建军,祖艳群,王吉秀.硫素对小花南芥体内含硫化合物与铅累积特征的影响[J].农业环境科学学报,2021,40(9):1851-1859. |
| 硫素对小花南芥体内含硫化合物与铅累积特征的影响 |
| Effects of sulfur on the characteristics of sulfur-containing compounds and Pb accumulation in Arabis alpina L. var. parviflora Franch |
| 投稿时间：2021-02-25 |
| DOI：10.11654/jaes.2021-0345 |
| 中文关键词: 硫素 Pb 富集系数 含硫化合物 酶活性 |
| 英文关键词: sulfur Pb bioconcentration factor sulfur-containing compounds enzyme activity |
| 基金项目:国家自然科学基金项目（41761073，41967049，41867055）；云南省教育厅科学研究基金项目（2020Y168） |
|
| 摘要点击次数: 1005 |
| 全文下载次数: 975 |
| 中文摘要: |
| 为探明含硫化合物促进小花南芥（Arabis alpina L.var.parviflora Franch）富集Pb的机理，采用外源添加不同硫浓度进行盆栽试验，通过测定谷氨酸、半胱氨酸、谷胱甘肽含量及酶活性，揭示超富集植物小花南芥体内含硫化合物对Pb累积特征的影响。结果表明：硫浓度为50 mg·kg-1处理下，小花南芥地上部、地下部生物量分别显著增加85.0%和46.2%；硫浓度为150、200 mg·kg-1处理下，土壤中有效态Pb含量分别显著增加3.6%和4.1%，小花南芥地下部Pb含量分别显著增加23.1%和32.8%；硫浓度为50、100 mg·kg-1处理促进了小花南芥对Pb的累积，富集系数分别显著增加64.2%和107.8%；硫处理显著提高了小花南芥体内含硫化合物含量及酶活性；小花南芥地下部Pb含量与地上部谷氨酸（Glutamine，Glu）含量呈极显著正相关，与地上部γ-谷氨酰半胱氨酸合成酶（γ-glutamylcysteine synthetase，γ-GCS）和谷胱甘肽还原酶（Glutathione reductase，GR）活性呈显著正相关；小花南芥地下部Pb含量与地下部半胱氨酸（Cysteine，Cys）含量呈极显著正相关，与地下部γ-GCS、GR和谷胱甘肽S-转移酶（Glutathione Stransferase，GST）活性呈显著正相关。研究发现，适量施硫能促进小花南芥体内含硫化合物的合成，提高关键酶活性和对Pb的富集能力，有利于对Pb污染土壤的修复。 |
| 英文摘要: |
| To explore the mechanism by which sulfur-containing compounds promote the accumulation of Pb in Arabis alpina L. var. parviflora Franch, a pot experiment was carried out with different sulfur concentrations added by exogenous sources to determine the content of cysteine, glutathione, and glutamine, and enzyme activity. The results showed that under the 50 mg·kg-1 S treatment, the shoot and root biomass of A. alpina significantly increased by 85.0% and 46.2%, respectively. Under the 150 and 200 mg·kg-1 S treatments, the available Pb content in the soil significantly increased by 3.6% and 4.1%, respectively, and the Pb content in the roots of A. alpina significantly increased by 23.1% and 32.8%, respectively. 50 and 100 mg·kg-1 S treatments promoted the accumulation of Pb in A. alpina, and the bioconcentration factor significantly increased by 64.2% and 107.8%, respectively. Sulfur treatment significantly increased the content of sulfur-containing compounds and enzyme activity in A. alpina. The content of Pb in the roots of A. alpina was extremely significantly positively correlated with the content of glutamate(Glu) in the shoot, and was positively correlated with the shoot γ-glutamylcysteine synthase(γ-GCS) and glutathione reductase(GR) activities. The content of Pb in the root of A. alpina was extremely significantly positively correlated with the content of cysteine(Cys) in the root, and significantly positively correlated with the activities of shoot γ-GCS, GR, and glutathione S-transferase(GST). Studies have found that an appropriate amount of applied sulfur promote the synthesis of sulfur-containing compounds in A. alpina, improve the activity of key enzymes, and improve the ability to enrich Pb, which is beneficial for the restoration of Pb-contaminated soil. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
