| 浦喆,杨新瑶,于丹,张琦.酸适应降低鼠伤寒沙门氏菌在天然土壤中迁移能力[J].农业环境科学学报,2022,41(12):2705-2710. |
| 酸适应降低鼠伤寒沙门氏菌在天然土壤中迁移能力 |
| Acid adaptation reduces the transport ability of Salmonella typhimurium in natural soil |
| 投稿时间：2022-11-19 修订日期：2022-12-01 |
| DOI：10.11654/jaes.2022-1185 |
| 中文关键词: 沙门氏菌 酸适应性改变 表面性质 迁移 XDLVO模型 |
| 英文关键词: Salmonella acid adaptability change surface property transport XDLVO model |
| 基金项目:国家自然科学基金面上项目（42177406）；辽宁省“百千万人才工程”人选资助项目 |
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| 中文摘要: |
| 基于土柱迁移实验，本研究在酸性环境（pH=5）下研究了发生适应性变化的鼠伤寒沙门氏菌的迁移规律。实验结果表明，酸性环境会导致鼠伤寒沙门氏菌的粒径和胞外膜脂多糖（LPS）浓度增加，胞外聚合物（EPS）浓度降低。这些胞外膜结构的生理变化会引起细菌Zeta电位负电性降低，疏水性增加，进而改变沙门氏菌的迁移规律。柱实验的结果显示沙门氏菌在发生酸适应性改变后迁移能力下降，其原因在于酸适应降低了沙门氏菌与沙介质表面的静电排斥，增强了疏水吸附，两种机制均促进沙门氏菌在沙表面的沉积。XDLVO（extented Derjaguin-Landau-Verwey-Overbeek）曲线中能量势垒和第二最小值深度表明沙门氏菌在土壤沙表面的沉降为可逆过程。本研究结果表明，酸性环境胁迫下沙门氏菌表面生理特征的改变会抑制其在土壤中的迁移，从而降低酸性环境中沙门氏菌的传播风险。 |
| 英文摘要: |
| Based on a soil column transport experiment, this study investigated the transport mechanism of Salmonella typhimurium, which underwent adaptive changes in an acidic environment（pH=5）. The results showed that acidic environment increased the particle size and concentration of lipopolysaccharide（LPS）in the extracellular membrane of Salmonella typhimurium and decreased the concentration of extracellular polymer（EPS）. These physiological changes in the extracellular membrane structure decreased the electronegativity of bacterial Zeta potential and increase its hydrophobicity, thereby changing the transport mechanism of Salmonella. Column experiment results showed that the transport ability of Salmonella decreased after their adaptation to acid stress. The reason was that the acid adaptability reduced the electrostatic repulsion between Salmonella and the surface of the sand medium and enhanced their hydrophobic adsorption. Both mechanisms promoted the deposition of Salmonella on the sand surface. The energy barrier and the second minimum depth in the XDLVO（extended Derjaguin-Landau-Verwey-Overbeek）curve indicated that the settlement of Salmonella on the sand surface was a reversible process. The results of this study show that the changes in the surface physiological characteristics of Salmonella under acid stress would inhibit their transport in soil, thereby reducing the risk of Salmonella transmission in an acidic environment. |
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