| 张寅寅,黑多尔,刘玥婷,李鑫.荷兰进境百合种球中线虫的分离及分子鉴定[J].农业环境科学学报,2022,41(12):2805-2809. |
| 荷兰进境百合种球中线虫的分离及分子鉴定 |
| Isolation and molecular identification of nematodes intercepted from imported Netherlands′ lily bulbs |
| 投稿时间：2022-11-10 修订日期：2022-12-06 |
| DOI：10.11654/jaes.2022-1141 |
| 中文关键词: 荷兰 百合种球 线虫 分子鉴定 |
| 英文关键词: Netherlands lily bulb nematode molecular identification |
| 基金项目:“十四五”国家重点研发计划课题（2021YFD1400100） |
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| 中文摘要: |
| 随着荷兰百合种球的进口量逐年增加，其极易携带多种有害生物入境，线虫传入风险随之增大，危害国门生物安全，因此对其准确的检疫鉴定十分重要。本文采用形态学（改良贝曼漏斗法）结合分子生物学方法（DNA提取及PCR扩增）对百合种球中分离到的植物寄生线虫进行分离鉴定。最终鉴定出3个种类，分别为穿刺短体线虫（Pratylenchus penetrans）、毁芽滑刃线虫（Aphelenchoides blastophthorus）和燕麦真滑刃线虫（Aphelenchus avenae）。研究表明，在进境百合种球中分离到的线虫数量有限、幼虫较多，无法通过形态学准确鉴定，应结合分子生物学方法保证结果的准确性。 |
| 英文摘要: |
| The increasing import of the Netherlands′ lily bulbs over the years has meant introduction of a variety of pests into the country; and one such nematode pest has turned into risk, endangering the national biosafety. Quarantine and identification of nematodes are thus imperative. This paper provides reference for the quarantine of imported seedlings and flowers to various ports and the supervision of planting sites. In this study, morphology（Beeman funnel method）, combined with molecular biological methods（DNA extraction and PCR amplification）, were used to quarantine and identify plant parasitic nematodes isolated from lily bulbs. The results showed that three species of plant parasitic nematodes were found：Pratylenchus penetrans, Aphelenchoides blastophthorus, and Aphelenchus avenae. The results also indicated that the quantity of nematodes isolated from the imported lily bulbs was limited, with most being larvae, as is it difficult to accurately identify nematodes based on their morphology, this process needed to be supplemented by molecular biological methods to ensure the accuracy of the results. |
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