| 张鹏,刘玮,王朋云,王铁杆,钟晨辉,陶月良.砷在铜藻中的亚细胞分布及细胞壁吸附作用研究[J].农业环境科学学报,2023,42(2):291-298. |
| 砷在铜藻中的亚细胞分布及细胞壁吸附作用研究 |
| Subcellular distribution of arsenic in Sargassum horneri and the role of the cell wall in arsenic biosorption |
| 投稿时间：2022-07-18 |
| DOI：10.11654/jaes.2022-0721 |
| 中文关键词: 细胞亚组分 细胞壁改性 吸附动力学 多糖 傅里叶红外光谱 铜藻 |
| 英文关键词: cell subfraction cell wall chemical modification sorption kinetics polysaccharide FTIR Sargassum horneri |
| 基金项目:农业部藻类产业技术体系项目（CARS-50）；温州市科技特派员专项项目（X20210057） |
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| 中文摘要: |
| 为揭示铜藻（Sargassum horneri）细胞壁在砷（As）生物吸附中的作用，以铜藻幼苗为研究对象，测定了不同As胁迫浓度（50、100、150、200、250 μmol·L-1）下细胞亚组分中的As含量变化，并对细胞壁的吸附动力学、细胞壁多糖As吸附效果以及细胞壁多糖傅里叶红外光谱进行了分析研究。结果表明：在低浓度（50 μmol·L-1） As胁迫时，50.0%的As分布于细胞壁，明显高于其他组分；而在高浓度（≥150 μmol·L-1） As胁迫时，As优势分布由细胞壁转向细胞可溶组分，细胞壁中的As比例下降至36.4%~37.3%。随着As胁迫浓度升高，细胞壁As含量呈上升趋势，当As胁迫浓度超过100 μmol·L-1时，细胞壁As含量上升趋于平缓。细胞壁吸附动力学实验表明酯化改性和甲基化改性可降低细胞壁As吸附的平衡容量，其降幅分别为68.5%和42.9%，同时细胞壁多糖红外光谱分析结果也表明羟基、氨基、羧基、硫酸盐基等官能团为细胞壁As吸附提供了结合位点。细胞壁多糖体外吸附实验表明随着多糖浓度的升高，多糖As吸附率呈上升趋势。研究表明铜藻细胞壁具有重要的As吸附能力，其中多糖官能团是细胞壁吸附As的关键因素，随多糖含量的升高细胞壁可吸附更多的As。 |
| 英文摘要: |
| In order to reveal the mechanism of arsenic(As) biosorption of the cell wall of Sargassum horneri, we used S. horneri seedlings to determine the variations of As distribution in cell subfraction under different As stress concentrations(50, 100, 150, 200 μmol·L-1, and 250 μmol·L-1). Experiments on the kinetics of the cell wall biosorption, As sorption effect of the polysaccharides extracted from the cell wall, and FTIR analysis were also carried out. The results showed that 50% of As was distributed in the cell wall fraction which was significantly higher than in other cell subfractions under a low level of As stress(50 μmol·L-1). However, with increasing As stress(≥ 150 μmol·L-1), As was dominantly distributed in the soluble fraction instead of the cell wall, in which less deposition was observed(36.4%- 37.3%). Although there was no notable increase in the As content of the cell wall fraction when As stress concentration was above 100 μmol·L-1, the As content of the cell wall fraction tended to increase with increasing As stress overall. Results from the kinetic analysis showed that both the esterification and the methylation of the cell wall decreased the equilibrium capacity of As sorption, which declined by 68.5% and 42.9%, respectively. Moreover, FTIR analysis revealed that functional groups such as hydroxyl, amino, carboxyl, and sulphate on the polysaccharide of the cell wall potentially provided binding sites for As. Sorption experiment in vitro showed that the rate of As sorption gradually increased with the increasing concentration of polysaccharides extracted. Our findings suggest that the cell wall of S. horneri has an important biosorption capacity of As, in which the functional groups on polysaccharides play key roles, and the cell wall can adsorb more As with increasing of polysaccharide content. |
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