| 李志斐,谢 骏,郁二蒙,王广军,余德光,夏 耘,王海英,龚望宝,魏 南.基于Biolog-ECO技术分析杂交鳢和大口黑鲈高产池塘水体微生物碳代谢特征[J].农业环境科学学报,2014,33(1):185-192. |
| 基于Biolog-ECO技术分析杂交鳢和大口黑鲈高产池塘水体微生物碳代谢特征 |
| Carbon Metabolic Diversity of Microbial Communities in Intensive Ponds for Hybrid Snakehead and Largemouth Bass Based on Biolog-ECO Plates |
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| DOI：10.11654/jaes.2014.01.024 |
| 中文关键词: Biolog-ECO 碳源利用 池塘养殖 微生物群落代谢 杂交鳢 大口黑鲈 |
| 英文关键词: Biolog-ECO carbon utilization pond culture microbial community metabolism hybrid snakehead（Channa maculata♀×Channa arguss♂） largemouth bass（Micropterus salmoides） |
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| 中文摘要: |
| 为了研究华南地区高产池塘高峰养殖季节微生物群落碳代谢特征，运用Biolog-ECO技术，分析了华南地区杂交鳢（Channa maculata♀×Channa arguss♂）和大口黑鲈（Micropterus salmoides）两种养殖池塘的水体微生物群落功能多样性。结果表明：杂交鳢和大口黑鲈两种池塘不同水层多样性指数（Shannon-Wiener指数、Simpson指数和丰富度指数）存在一定程度的差异，但在使用增氧机条件下，每种池塘的上、中、底3水层间水体微生物活性（AWCD）均无显著性差异（P>0.05），且两种池塘间的AWCD为杂交鳢池塘强于大口黑鲈池塘；两种池塘水体微生物对6类碳源利用率规律相似，碳源的代谢利用率由强到弱为碳水化合物类>聚合物类>氨基酸类>羧酸类>胺类>酚类。水体微生物代谢特征PCA分析表明，两种池塘间的样方分布在不同区域，但池塘间样点间离散程度较大，表明两种池塘水体微生物存在一定的代谢差异性。决定两种池塘间代谢差异的碳源有：D-半乳糖酸内脂（D-Galactonic Acid Lactone）>D-纤维二糖（D-Cellobiose）>肝糖（Glycogen）>D-木糖（D-Xylose）> L-丝氨酸（L-Serine）。Biolog-ECO技术可用于分析养殖水体微生物群落碳代谢差异，以及造成代谢差异的决定因素，同时可有效识别容易被养殖水体微生物利用的碳源类型。 |
| 英文摘要: |
| Carbon types usually affect microbial carbon metabolism. Carbon metabolic diversity of bacteria in intensive ponds for hybrid snakehead（Channa maculata♀×Channa arguss♂） and largemouth bass（Micropterus salmoides） was investigated using the Biolog-ECO plates. The Shannon-Wiener index， Simpson index and richness index were different between two ponds and different water depths. There were no differences for average well color development（AWCD） in water layers of each pond with an aerator， but AWCD was higher in the ponds of hybrid snakehead than of largemouth bass. The most utilized carbon sources by microorganisms were similar in both ponds， and were in order of carbohydrates > Polymers > Amino acids > Carboxylic acids > Phenolic compounds > Amines. The principal components analysis showed that those carbon determining microbial utilization differences in two ponds were as follows：D-Galactonic Acid Lactone > D-Cellobiose > Glycogen > D-Xylose > L-Serine. The results demonstrate that Biology-ECO plates could be useful in differentiating carbon metabolic diversity between microbial communities of fishery water and identifying factors that most influence community variation and substrates that are most utilized by the communities in aquaculture ponds. |
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