文章摘要
王文娜,王沛芳,常 虹,尹亚云,王永泉,张微敏.硒在河蚬体内的富集及其对河蚬的氧化应激[J].农业环境科学学报,2014,33(6):1118-1123.
硒在河蚬体内的富集及其对河蚬的氧化应激
Selenium Bioaccumulation and Its Influence on Antioxidant System in Corbicula fluminea
  
DOI:10.11654/jaes.2014.06.010
中文关键词:   河蚬  富集  氧化应激
英文关键词: selenium  Corbicula fluminea  bioaccumulation  oxidative stress
基金项目:
作者单位
王文娜 河海大学 浅水湖泊综合治理与资源开发教育部重点实验室 河海大学环境学院 南京 210098 
王沛芳 河海大学 浅水湖泊综合治理与资源开发教育部重点实验室 河海大学环境学院 南京 210098 
常 虹 河海大学 浅水湖泊综合治理与资源开发教育部重点实验室 河海大学环境学院 南京 210098 
尹亚云 江苏省水利工程规划办公室 南京 210029 
王永泉 河海大学 浅水湖泊综合治理与资源开发教育部重点实验室 河海大学环境学院 南京 210098 
张微敏 河海大学 浅水湖泊综合治理与资源开发教育部重点实验室 河海大学环境学院 南京 210098 
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中文摘要:
      通过实验室模拟的方法,以河蚬(Corbicula fluminea)为实验生物,研究了太湖沉积物中硒在河蚬体内的富集及其对河蚬抗氧化系统和过氧化产物的影响。结果显示:硒在河蚬体内的富集与沉积物中硒浓度呈线性相关,生物富集系数(Bioconcentration factor, BCF)与暴露浓度之间呈幂函数关系;随着硒暴露浓度的增大,河蚬受毒害,内脏团颜色加深;硒对河蚬内脏团的几种酶活性和过氧化产物表现出不同程度的影响。酶活性随硒浓度增大均表现为先升高后降低的趋势,其中谷胱甘肽过氧化物酶(GPx)和过氧化氢酶(CAT)活性对硒的响应比超氧化物歧化酶(SOD)敏感,最大响应浓度均为0.609 mg·kg-1,分别为对照组的2.84倍和4.82倍,而小于0.609 mg·kg-1和1.007 mg·kg-1的暴露浓度可显著增强CAT和GPx活性。在整个实验浓度范围内,硒对SOD活性基本都表现为增大作用,SOD活性在硒暴露浓度为1.638 mg·kg-1时具有最大响应值,是对照组的2.87倍,丙二醛(MDA)含量在1.638 mg·kg-1时也具有最大响应值,但MDA的响应较复杂,在本研究中未表现出明显的响应趋势。河蚬对不同硒浓度的响应说明河蚬可作为沉积物中硒污染环境监测的有效指示生物。
英文摘要:
      Corbicula fluminea has showed potential to be a bio-indicator of sediment pollution. A laboratory experiment was undertaken to investigate the bioaccumulation of selenium(Se) and its influence on antioxidant system and peroxidation product in the visceral mass of C. fluminea under exposure of 14 days. Sediments, obtained from Lake Taihu, were spiked with sodium selenite. The bioaccumulation of Se in C. fluminea was linearly related to Se concentrations in the sediments. The bio-concentration factor(BCF) had a power function relationship with Se concentrations. The color of the visceral mass of C. fluminea gradually became blackened with increasing Se concentrations. The activities of catalase(CAT), glutathione peroxidase(GPx) and superoxide dismutase(SOD) showed “increase and then decrease” pattern as Se concentrations increased. GPx and CAT were more sensitive to Se contamination than SOD. The maximum activities of CAT and GPx were observed both at 0.609 mg·kg-1 of Se exposure concentration, being 2.84 times and 4.82 times of the control. SOD activity was greatest at 1.638 mg Se·kg-1, which was 2.87 times of the control. The contents of MDA didn′t show consistent responses to Se concentrations. These results indicate that C. fluminea could be used as an indicator of Se pollution in water sediments.
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