| 曹俊超,王小庆,马保华,邹永德,廖新俤,庞燕玲,吴银宝.不同加入方式下金霉素对猪场污水厌氧消化系统的影响[J].农业环境科学学报,2019,38(3):680-687. |
| 不同加入方式下金霉素对猪场污水厌氧消化系统的影响 |
| Comparison of anaerobic digestion systems in swine wastewater treatment with different chlortetracycline addition methods |
| 投稿时间：2018-04-27 |
| DOI：10.11654/jaes.2018-0555 |
| 中文关键词: 金霉素 厌氧消化 猪场污水 产甲烷量 产甲烷菌多样性 |
| 英文关键词: chlortetracycline anaerobic fermentation swine wastewater methane production methanogen diversity |
| 基金项目:国家自然科学基金项目（31872401）；广东省现代农业产业共性技术创新团队建设项目（2016LM2152） |
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| 摘要点击次数: 1897 |
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| 中文摘要: |
| 采用两种金霉素（CTC）加入方式，即经体内代谢法（系统中加入经饲喂金霉素后所获含金霉素的猪粪）和直接添加法（系统中加入空白猪粪+金霉素），两种CTC加入浓度为310.54 mg·kg-1干猪粪（高浓度）和160.05 mg·kg-1干猪粪（低浓度），研究了金霉素对猪场污水厌氧消化系统影响，并比较分析了两种研究方法间的差异。试验在有效容积为1.3 L的模型厌氧消化系统中进行，恒温30℃，连续7 d加入含金霉素浓度为310.54 mg·kg-1和160.05 mg·kg-1的猪粪，检测了加药期和停药期金霉素及其活性代谢产物4-差向异构金霉素浓度、系统产甲烷量、pH、产甲烷菌多样性等指标。结果表明，金霉素加入浓度为310.54 mg·kg-1的直接添加组中金霉素浓度显著高于经体内代谢组（P<0.05），4-差向异构金霉素浓度则显著低于经体内代谢组（P<0.05）；与经体内代谢组相比，加药期内两种浓度的直接添加组产甲烷量分别显著降低了4.40%、5.22%（P<0.05），而且直接添加组中沼液pH显著低于经体内代谢组和空白对照组（P<0.05）；两种研究方法均未显著影响厌氧消化系统产甲烷菌多样性（P>0.05）。由此可知，采用直接添加法时，加入310.54 mg·kg-1的金霉素会导致厌氧消化系统中金霉素大量累积，并显著降低沼液pH和抑制加药期内厌氧消化系统的产甲烷量。但采用经体内代谢法时金霉素的加入未显著影响系统产甲烷量。因此，在本试验条件下，两种研究方法所得金霉素对猪场污水厌氧消化系统的影响不同，但经体内代谢法能更好地反映生产实际，获得更准确的研究结果。 |
| 英文摘要: |
| This study examined the effect of chlortetracycline (CTC) addition methods (using manure collected from swine fed with CTC or adding CTC directly) on anaerobic digestion systems in swine wastewater treatment under two different CTC concentrations (310.54 mg·kg-1 DM and 160.05 mg·kg-1 DM) using 1.3 L anaerobic reactors run at 30℃ for 7 consecutive days. Concentrations of CTC and its active metabolite 4-epi-CTC, methane production, pH, and methanogen diversity were examined. The results showed that the concentration of CTC in the direct addition group, with an initial CTC concentration of 310.54 mg·kg-1, was significantly higher than that of the in vivo group (P<0.05). Meanwhile, the concentration of 4-epi-CTC in the latter was significantly higher than that in the former (P<0.05). Methane production in the direct addition group was reduced by 4.40% and 5.22%, respectively, at the two concentrations, compared to the in vivo group (P<0.05). The pH of the biogas slurry in the direct addition method group was significantly lower than that in the in vivo and blank groups (P<0.05). Both addition methods had no significant effect on the diversity of methanogens in the anaerobic digestion system (P>0.05). The direct addition of 310.54 mg·kg-1 CTC led to high CTC accumulation in the anaerobic digestion system, significantly reduced the pH of the biogas slurry, and inhibited the amount of methane produced. Therefore, the effects of different CTC addition methods on the anaerobic digestion system in swine wastewater treatment were different under the experimental conditions, and the use of an in vivo method might reflect actual production and provide more accurate results. |
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