| 张迪,厉圆,沈忱思,肖冬雪,柳建设,赵凤,熊明瑜.金霉素及其异构体降解产物对斜生栅藻的毒性效应研究[J].农业环境科学学报,2019,38(4):756-764. |
| 金霉素及其异构体降解产物对斜生栅藻的毒性效应研究 |
| Understanding the toxic effects of chlortetracyclineandits and its isomer degradation products on Scenedesmus obliquus |
| 投稿时间：2018-07-25 |
| DOI：10.11654/jaes.2018-0964 |
| 中文关键词: 金霉素 异构体 斜生栅藻 降解产物 毒性效应 |
| 英文关键词: chlortetracycline (CTC) isomers Scenedesmus obliquus degradation products toxicity |
| 基金项目:国家自然科学基金项目（21777023）；国家重点研发计划项目（2016YFC0400501）；东华大学中央高校基金项目（2232016D-12）；上海市青年科技英才扬帆计划项目（17YF1425600）；中央级公益性科研院所基本业务费专项资金（2016T10）；中央高校基金项目（2232018G-11） |
| 作者 | 单位 | E-mail | | 张迪 | 东华大学环境科学与工程学院, 国家环境保护纺织工业污染防治工程技术中心, 上海 201620 | | | 厉圆 | 东华大学环境科学与工程学院, 国家环境保护纺织工业污染防治工程技术中心, 上海 201620 | | | 沈忱思 | 东华大学环境科学与工程学院, 国家环境保护纺织工业污染防治工程技术中心, 上海 201620
上海污染控制与生态安全研究院, 上海 200092 | shencs@dhu.edu.cn | | 肖冬雪 | 中国水产科学研究院东海水产研究所, 上海 200090 | | | 柳建设 | 东华大学环境科学与工程学院, 国家环境保护纺织工业污染防治工程技术中心, 上海 201620 | | | 赵凤 | 东华大学环境科学与工程学院, 国家环境保护纺织工业污染防治工程技术中心, 上海 201620 | | | 熊明瑜 | 嘉兴市环境科学研究所有限公司, 浙江 嘉兴 314100 | |
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| 中文摘要: |
| 为探索水体中四环素类抗生素异构体降解产物的毒性效应，选取金霉素及其异构体降解产物为目标化合物，斜生栅藻（Scenedesmus obliquus）为受试生物，结合藻类生理指标探讨金霉素及其异构体降解产物的毒性效应。研究结果表明，在斜生栅藻藻液中，金霉素主要的异构体降解产物为异金霉素及异差向金霉素。暴露72 h后，金霉素及其异构体降解产物处理组的藻细胞形态发生了明显的质壁分离，且细胞通透性均显著增大。但是，金霉素母体药物与不同异构体降解产物对斜生栅藻的毒性效应表现出了明显的差异。金霉素母体药物处理组的斜生栅藻细胞中可溶性蛋白质和叶绿素a浓度降低得更为显著，且氧化损伤更为严重。金霉素母体药物与其异构体降解产物虽然具有相似的化学结构，但取代基空间构象的不同会导致药物与藻细胞中的可溶性蛋白质的结合位点不同，因而对藻细胞产生不同的毒性效应。探究金霉素及其异构体降解产物对水生环境产生的毒性效应，有望为全面认识四环素类抗生素的生态环境风险提供新的依据。 |
| 英文摘要: |
| To explore the toxicity of tetracycline antibiotics and their isomeric degradation products in the aqueous environment, chlortetracycline was used as the target compound and Scenedesmus obliquus as the test organism. A study of the growth inhibitors and physiological indicators of the algae was combined with an investigation of the difference in toxicity between chlortetracycline and its isomers. The results showed that the degradation products of chlortetracycline were mainly iso-chlortetracycline and epi-iso-chlortetracycline in the presence of Scenedesmus obliquus. After being exposed to chlortetracycline and its isomeric degradation products for 72 h, the Scenedesmus obliquus experienced plasmolysis and its cell permeability increased significantly. In addition, the difference in toxicity between chlortetracycline and its isomeric degradation products was obvious. In the chlortetracycline treatment, the concentration of soluble protein and chlorophylla in the Scenedesmus obliquus decreased significantly and it underwent serious oxidative damage. Although chlortetracycline and its isomeric degradation products had similar chemical structures, they did not have the same toxic effects on algal cells. This might be due to differences in the conformation of the substituents in chlortetracycline and its isomers, which would cause the drugs to bind to different sites on the proteins in the algal cells. It is hoped that this investigation of the toxic effect of chlortetracycline and its isomeric degradation products on an aquatic organism will be help provide a deeper understanding of the environmental risks of tetracycline antibiotics. |
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