| 唐蕊,邵红,贾春云,张作金,陈祥.苯并[a]芘对毛霉EPS特征的影响[J].农业环境科学学报,2019,38(4):765-772. |
| 苯并[a]芘对毛霉EPS特征的影响 |
| Effect of benzo[a]pyrene on the characteristics of Mucor mucedo EPS |
| 投稿时间：2018-07-03 |
| DOI：10.11654/jaes.2018-0852 |
| 中文关键词: 胞外聚合物 苯并[a]芘 毛霉 理化性质 变化特征 |
| 英文关键词: extracellular polymer benzo[a]pyrene M. mucedo physicochemical property variation characteristic |
| 基金项目:国家自然科学基金项目（41673132，41201310） |
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| 摘要点击次数: 1938 |
| 全文下载次数: 1551 |
| 中文摘要: |
| 为了明确毛霉胞外聚合物（Extracellular polymertic substances，EPS）在多环芳烃修复中的作用机理，研究了不同浓度苯并[a]芘（BaP）诱导下毛霉EPS的提取量（TOC）和主要化学成分含量（糖类、蛋白质、腐植酸和DNA）的变化规律，采用三维荧光光谱（3D-EEM）、傅里叶红外光谱（FTIR）和扫描电镜（SEM）等方法分析了不同条件下EPS蛋白含量和自身结构的变化。结果表明，当BaP浓度为40 mg·L-1时，单位生物量所产生的毛霉EPS的提取量、糖类含量、蛋白质含量和腐植酸含量均达到最大值，分别为1 407.22 mg·g-1、700 g·g-1、564 g·g-1和174 g·g-1；3D-EEM结果显示，当BaP浓度为40 mg·L-1时，荧光类蛋白质（峰A和峰B）强度分别达到最大值68.91 a.u和245.6 a.u；FTIR分析结果表明，在BaP的诱导下，与空白相比，EPS的细胞质蛋白质酰胺Ⅰ带和酰胺Ⅱ带向长波方向发生了红移现象；SEM分析结果显示，随BaP浓度的增大，毛霉EPS表面逐渐呈现板结现象，当BaP浓度为40 mg·L-1时，毛霉EPS呈毛绒状，孔隙达到最大。以上分析结果表明，BaP破坏毛霉EPS原有结构，低浓度BaP促进真菌EPS的分泌，EPS中的蛋白质和多糖在毛霉代谢降解BaP过程中起到了主要作用。 |
| 英文摘要: |
| To elucidate the role of Mucor mucedo EPS in the remediation of polycyclic aromatic hydrocarbon (PAH) pollution, the extracted concentration of EPS (TOC) and the content of proteins (PN), polysaccharides (PS), humic acid (HC), and DNA in EPS were determined after the exposure of M. mucedo to different concentrations of benzo[a]pyrene (BaP). Three-dimensional fluorescence spectroscopy (3DEEM) and flourier transform infrared spectroscopy (FTIR) were used to verify the changes in the concentration of proteins when different concentrations of BaP were used, and scanning electron microscopy (SEM) was employed to study the changes in the structure of EPS. The results showed that the changes in the concentration of TOC, PN, PS, and HC showed the same trend as the exposed BaP concentrations increased, and their content increased initially, and then decreased. When the concentration of BaP was 40 mg·L-1, the concentration of TOC, PN, PS, and HC produced by unit biomass reached the maximum values of 1 407.2 mg·g-1, 700 g·g-1, 564 g·g-1, and 174 g·g-1, respectively. According to the results of 3D-EEM, when the concentration of BaP was 40 mg·L-1, the intensity of fluorescence protein (peak A and peak B) reached the maximum value of 68.91 a.u and 245.6 a.u. The analysis of FTIR showed that compared with that of the blank control, the cytoplasmic protein amideⅠand amide Ⅱ bands of the EPS shifted to the long wave direction under the induction of BaP. The results of SEM indicated that the increase in BaP concentration gradually hardened the EPS surface, the EPS was wool-like, and the voids reached the maximum number when the concentration of BaP was 40 mg·L-1. These findings show that BaP can break the original structure of M. mucedo EPS, low concentrations of BaP can promote the production of fungus EPS, and PN and PS might play a major role during the metabolic degradation of BaP by M. mucedo. |
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