| 黄哲,邵杰,曾昆,张旭芸,陈斌,杜道林.四溴双酚A类阻燃剂的酶联免疫方法的构建及应用[J].农业环境科学学报,2020,39(6):1420-1428. |
| 四溴双酚A类阻燃剂的酶联免疫方法的构建及应用 |
| Construction and application of an enzyme-linked immunosorbent assay for detection of tetrabromobisphenol A |
| 投稿时间：2020-01-17 |
| DOI：10.11654/jaes.2020-0079 |
| 中文关键词: TBBPA TBBPA-DHEE TBBPA-MHEE 单克隆抗体 |
| 英文关键词: TBBPA TBBPA-DHEE TBBPA-MHEE monoclonal antibody |
| 基金项目:国家自然科学基金项目（31502118，21577051） |
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| 摘要点击次数: 2230 |
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| 中文摘要: |
| 为建立快速、灵敏的检测方法评估四溴双酚A（Tetrabromobisphenol A，TBBPA）类阻燃剂四溴双酚A双（2-羟基乙基）醚（TBBPA-DHEE）在环境中的行为分布，本研究基于TBBPA-DHEE的结构，设计并合成半抗原D3；采用杂交瘤技术制备了5株针对TBBPA-DHEE的单克隆抗体；鉴定发现这些抗体均与四溴双酚A单（2-羟乙基）醚（TBBPA-MHEE）有较高的交叉反应，与其他TBBPAs没有交叉反应；优化抗原/抗体工作浓度、缓冲液条件后，建立了间接竞争ELISA标准曲线。结果显示优化后的ELISA方法检测TBBPA-MHEE的检测范围为0.86~13.70 ng·mL-1，检测限（LOD）为0.78 ng·mL-1；检测TBBPA-DHEE的检测范围为0.96~8.10 ng·mL-1，检测限为0.56 ng·mL-1。采集山东省寿光市一家BFR工厂周边的水样和土壤样本，发现TBBPA-DHEE/TBBPAMHEE在水样中浓度最高可达15.45 ng·mL-1，土壤样本中浓度最高可达6.75 ng·g-1。 |
| 英文摘要: |
| A rapid and sensitive detection method was established in this study for evaluating the environmental behavior of tetrabromobisphenol A bis(2-hydroxyethyl)ether(TBBPA-DHEE). On the basis of its structure, hapten D3 was designed and synthesized, and five monoclonal antibodies against TBBPA-DHEE were prepared using hybridoma technology. These antibodies showed high cross-reactivity with tetrabromobisphenol A mono(2-hydroxyethyl)ether(TBBPA-MHEE)and no cross-reactivity with other TBBPAs. After the reaction conditions were optimized, the results showed that the linear ranges of TBBPA-MHEE and TBBPA-DHEE were 0.86~13.70 ng·mL-1 and 0.96~8.10 ng·mL-1 with detection limits of 0.78 ng·mL-1 and 0.56 ng·mL-1, respectively. Moreover, in water and soil samples collected near a brominated flame retardants(BFR)plant in Shouguang City, Shandong Province, the maximum concentrations of TBBPA-DHEE/TBBPA-MHEE were 15.45 ng·mL-1 and 6.75 ng·g-1, respectively. |
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