文章摘要
付宇童,孙彤,王林,徐应明,梁学峰,孙约兵.纳米铜对小油菜(Brassica chinensis L.)种子发芽和幼苗生理生化特性的影响[J].农业环境科学学报,2020,39(11):2524-2531.
纳米铜对小油菜(Brassica chinensis L.)种子发芽和幼苗生理生化特性的影响
Effects of copper nanoparticles on seed germination and physiological and biochemical characteristics of Brassica chinensis L.
投稿时间:2020-02-24  
DOI:10.11654/jaes.2020-0189
中文关键词: 纳米铜  抑制率  Cu含量  发芽  酶活性
英文关键词: copper nanoparticles  inhibition rate  Cu content  germination  enzyme activity
基金项目:国家自然科学基金面上项目(31971525);中国农业科学院基本科研业务费专项院级统筹项目(Y2020PT03)
作者单位E-mail
付宇童 东北农业大学资源与环境学院, 哈尔滨 150030
农业农村部环境保护科研监测所, 农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室, 天津 300191 
 
孙彤 东北农业大学资源与环境学院, 哈尔滨 150030
农业农村部环境保护科研监测所, 农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室, 天津 300191 
 
王林 农业农村部环境保护科研监测所, 农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室, 天津 300191  
徐应明 农业农村部环境保护科研监测所, 农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室, 天津 300191  
梁学峰 农业农村部环境保护科研监测所, 农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室, 天津 300191  
孙约兵 农业农村部环境保护科研监测所, 农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室, 天津 300191 sunyuebing@aepi.org.cn 
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中文摘要:
      通过纸床培养,探究了不同剂量(1、2、4、6、8、10 mg·L-1)纳米铜(Copper nanoparticles,Cu NPs)对小油菜(Brassica chinensis L.)种子发芽、幼苗Cu含量、根细胞微观特征以及幼苗生理生化特性的影响。结果表明,不同浓度Cu NPs处理对种子发芽率无显著影响(P>0.05),小油菜根和茎伸长量均随Cu NPs浓度增加先升高后降低,当Cu NPs添加量为2~10 mg·L-1时小油菜根伸长抑制率为10.7%~59.9%,而当Cu NPs≥4 mg·L-1时小油菜茎长显著低于对照组(P<0.05),降低了4.8%~15.8%。幼苗体内Cu含量随着Cu NPs浓度的增加而升高。通过透射电子显微镜可以看出,添加10 mg·L-1 Cu NPs使根细胞出现比较普遍的质壁分离现象。与对照组比较,Cu NPs处理后小油菜幼苗体内超氧化物歧化酶活性降低了4.5%~64.0%(1 mg·L-1除外),过氧化物酶降低幅度达到4.4%~59.3%,而不同浓度Cu NPs处理对小油菜幼苗体内过氧化氢酶活性影响不显著(P>0.05),因此,小油菜生理生化特性对不同剂量Cu NPs响应不同。
英文摘要:
      A paper-bed culture experiment was conducted to investigate the effects of different dosages (1, 2, 4, 6, 8 mg·L-1, and 10 mg·L-1)of copper nanoparticles (Cu NPs)on Brassica chinensis L., including seed germination, Cu content in seedlings, the microscopic characteristics of root cells, and physio-chemical characteristics. The results showed that Cu NPs had no significant effect on the seed germination rate (P>0.05). The root and shoot elongation of Brassica chinensis L. increased first and then decreased with the increase of Cu NPs content. The inhibition rate of Brassica chinensis L. root elongation was 10.7%~59.9% under the treatment of 2~10 mg·L-1 Cu NPs. The inhibition rate of Brassica chinensis L. shoots was significantly decreased when the amount of Cu NPs reached 4 mg·L-1, exhibiting a 4.8%~15.8% reduction in contrast with the control. The Cu content in Brassica chinensis L. seedlings increased with the increase of Cu NPs concentration. Transmission electron microscopy showed that the addition of 10 mg·L-1 Cu NPs resulted in a common wall separation of root cells. Compared with the control group, the activity of superoxide dismutase decreased by 4.5%~64.0% under the treatments of Cu NPs (except for 1 mg·L-1 Cu NPs); the peroxidase activity was inhibited by 4.4%~59.3%. However, catalase activity had no significant differences among the addition of Cu NPs (P>0.05). Therefore, the physiological and biochemical characteristics of Brassica chinensis L. vary in response to the doses of Cu NPs.
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