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| Analysis of Cultivable Microbial Community Structure and Affiliation in Saline- Alkaline Soil in the Tuanbo Lake Area of Tianjin, China |
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| KeyWord:haloalkaliphilus；16S rDNA；phylogenetic analysis；bacterial diversity |
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| Abstract: |
| In order to reveal the diversity of cultivable haloalkaliphilus from the Tuanbo Lake Area of Tianjin, we isolated cultivable bacteria using the Horikoshi Ⅱ medium with varying NaCl concentration（0~15%） and pH（7~9.5）. A total of seventy-five halophilic bacteria were isolated from the Tuanbo Lake area. All isolated bacteria were subjected to the 16S rDNA sequence analysis. Five genera including Bacillus, Exiguobacterium, Micrococcus, Oceanobacillus, and Jeotgalibacillus were identified. Bacillus and Oceanobacillus are the dominate genera, respectively accounting for 48% and 24% of all isolated bacteria. Based on the similarity of isolated bacteria to known sequences in GenBank（16S rDNA gene sequence similartities ranged from 99.6% to 99.9%）, at least four strains（TJTB06, TJTB32, TJTB50 and TJTB51） represent potentially new strains. The results of the study showed that cultivable haloalkaliphilus bacterial populations are quite abundant in the Tuanbo Lake area. It was clear that these haloalkaliphilus have adapted to the special man-made hypersaline environment by basic physiological evolution during phylogenesis, so they could be determined according to further polyphasic taxonomy data. The current results also indicated a phenotypically and genotypically diverse culture-dependent haloalkaliphilus bacterial community colonizing the Tuanbo Lake area. They provide potentially useful microbial resources, which require further in-depth investigation. |
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