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Effects of Exogenous AsA on the Growth of Dianthus chinensis Seedlings Under Soil Cd Stress
  
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KeyWord:soil; Dianthus chinensis seedlings; AsA; Cd stress; alleviating effect
Author NameAffiliation
DING Ji-jun College of Landscape Architecture, Sichuan Agricultural University
The Three Gorges Industrial Co., Ltd 
PAN Yuan-zhi College of Landscape Architecture, Sichuan Agricultural University 
LIU Shi-liang College of Landscape Architecture, Sichuan Agricultural University 
HE Yang College of Landscape Architecture, Sichuan Agricultural University 
WANG Li College of Landscape Architecture, Sichuan Agricultural University 
LI Li College of Forestry, Southwest Forestry University 
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Abstract:
      In order to investigate alleviating effects of exogenous ascorbic acid(AsA) for Dianthus chinensis seedlings suffering cadmium stress, a greenhouse pot experiment was conducted to explore the effects of different concentrations(0, 20, 40, 60, 80 mg·L-1 and 100 mg·L-1)of exogenous AsA on the growth of Dianthus chinensis seedlings. Cd(50 mg·kg-1) significantly inhibited the growth of Dianthus chinensis seedlings. Spraying exogenous AsA(≤40 mg·L-1) markedly alleviated the stress effect of Cd. Exogenous AsA significantly increased the activities of the catalase(CAT), peroxidase(POD), ascorbate peroxidase(APX), monodehydroascorbate reductase(MDAR), dehydroascorbate reductase(DHAR) and glutathione reductase(GR). Compared with Dianthus chinensis seedlings under Cd stress, exogenous AsA also significantly increased the biomass, plant height, number of tillers, and the contents of AsA and GSH. Exogenous AsA markedly reduced the cell membrane permeability, the shoots Cd content, production rates and accumulative amount of H2O2. However, the alleviating effect of exogenous AsA was showed as quadratic curve. With higher concentration, the exogenous AsA showed pro-oxidant effects. The present study indicated that the optimal concentration of exogenous AsA was 35~45 mg·L-1 to alleviate the Cd stress.