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| Isolation and Identification of Aerobic Polychlorinated Biphenyls-Degrading Bacterial Strains in PCBs-contaminated Soils |
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| KeyWord:polychlorinated biphenyl; aerobic degradation; isolation and identification |
| Author Name | Affiliation | | TANG Wei | MOE Key Lab of Environmental Remediation and Ecosystem Health, College of Environmental and Resources Science, Zhejiang University | | LI Ying | MOE Key Lab of Environmental Remediation and Ecosystem Health, College of Environmental and Resources Science, Zhejiang University | | LIANG Lu-yi | MOE Key Lab of Environmental Remediation and Ecosystem Health, College of Environmental and Resources Science, Zhejiang University | | ZHU You-feng | MOE Key Lab of Environmental Remediation and Ecosystem Health, College of Environmental and Resources Science, Zhejiang University | | WANG Feng-ping | MOE Key Lab of Environmental Remediation and Ecosystem Health, College of Environmental and Resources Science, Zhejiang University |
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| Abstract: |
| Growth of microorganisms was stimulated by incubating polychlorinated biphenyl-polluted soils in Wenling and Xiaoshan with biphenyl as sole carbon and energy source. After 4 weeks, a total of thirty five strains of bacteria which were able to grow on biphenyl-containing minimal medium plates were isolated. Sixty percent of them are gram-negative bacteria. The results of 16S rRNA gene sequencing showed that the dominant strains are Stenotrophomonas sp. and Pandoraea sp.. We chose two well growing strains from each soil to further study PCB-degradation. The four strains are Corynebacterium sp. WL8, Bacillus sp. WL10, Microbacterium sp. XS4 and Citrobacter sp. XS7. All of them were cultured in fresh mineral medium containing 100 mg·L-1 biphenyl and 2 mg·L-1 2, 3, 4, 5-tetrachlorobiphenyl（PCB61） for five days. The residual concentrations of PCB61 determined by gas chromatography showed all the strains could degrade PCB61. Especially,the transformation rate of PCB61 by Corynebacterium sp.WL 8, isolated from Wenling soil, reached 50%.This result will benefit further research of genetic screening, cloning and restructuring, as well as providing guidance for the cultivation of PCBs-degrading bacteria. |
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