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| Subcellular Distribution and Chemical Forms of Cd and the Synthesis of Phytochelatins（PCs） in Different Barley Genotypes |
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| KeyWord:barley; cadmium; subcellular distribution; chemical form; phytochelatins |
| Author Name | Affiliation | | LU Zhong-yan | Centre for Research in Ecotoxicology and Environmental Remediation, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China
Key Laboratory of Production Environment and Agro-Product Safety, Ministry of Agriculture, Tianjin 300191, China | | LIU Zhong-qi | Centre for Research in Ecotoxicology and Environmental Remediation, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China
Key Laboratory of Production Environment and Agro-Product Safety, Ministry of Agriculture, Tianjin 300191, China | | SONG Zheng-guo | Centre for Research in Ecotoxicology and Environmental Remediation, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China
Key Laboratory of Production Environment and Agro-Product Safety, Ministry of Agriculture, Tianjin 300191, China | | ZHANG Chang-bo | Centre for Research in Ecotoxicology and Environmental Remediation, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China
Key Laboratory of Production Environment and Agro-Product Safety, Ministry of Agriculture, Tianjin 300191, China | | SUN Dong-fa | College of Plant Technology of Huazhong Agricultural University,Wuhan 430070, China |
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| Abstract: |
| Cadmium has similar accumulation in different genotypes, but these genotypes suffer from different Cd toxicity. A hydroponic experiment was carried out to study the subcellular distribution and chemical forms of Cd in plant roots and shoots using a Cd-tolerant variety Hua10118 and a Cd-sensitive variety Hua2255. The effects of phytochelatins（PCs） in the roots and leaves on the Cd transport rate were also examined. Experimental results showed that Cd transport rates and Cd concentrations in shoots of Hua10118 were lower than those of Hua2255. Cadmium distributed mainly in cell wall and cytosol in both varieties, each accounting for 35%~44%. Cadmium in the organelles was only 5%~26%. Cadmium concentration in cell organelles of hua2255 was higher than that of hua10118，probably resulting in more sensitivity of hua2255 to Cd. The predominant form of Cd in the roots was HAc-extractable Cd, but NaCl-extractable Cd in the shoots. The amounts of ethanol-extractable, water-extractable and NaCl-extractable Cd were higher in hua2255 than that in hua10118, leading to different Cd transport and tolerance between two varieties. The concentrations of PC2 and PC3 were remarkably higher in the roots and shoots of hua2255 than in hua10118, implying that synthesis of more PC2 and PC3 in root facilitated Cd transfer to shoot. |
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