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| Screening and Fermentation Optimization of a Biosurfactant-producing Oil-degrading Bacterium |
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| KeyWord:biosurfactant; oil-degrading bacteria; fermentation optimization; Pseudomonas sp.1 |
| Author Name | Affiliation | | ZHANG Hui | The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education/School of Environment and Energy, South China University of Technology, Guangzhou 510006, China | | GUO Chu-ling | The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education/School of Environment and Energy, South China University of Technology, Guangzhou 510006, China | | LU Gui-ning | The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education/School of Environment and Energy, South China University of Technology, Guangzhou 510006, China | | YANG Chen | The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education/School of Environment and Energy, South China University of Technology, Guangzhou 510006, China | | DANG Zhi | The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education/School of Environment and Energy, South China University of Technology, Guangzhou 510006, China | | WU Ren-ren | The Key Laboratory of Water and Air Pollution Control of Guangdong Province/South China Institute of Environmental Sciences, Ministry of Environmental Protection, Guangzhou, 510655, China |
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| Abstract: |
| The aim of the present study was to isolate oil-degrading bacterial strains with high ability to produce biosurfactants. Bacterial strains from oil contaminated soil near a refinery were enriched and incubated with oil as the sole carbon source. Bacterial strains to produce biosurfactants were then screened by testing oil collapse-dropping, emulsification index and surface tension, and those strains with the ability to degrade oil were further screened by DCPIP tests. An oil-degrading bacterial strain MZ01 with high ability to produce biosurfactants was obtained. It was identified as Pseudomonas sp. MZ01 by gram-staining, morphology, and 16S rDNA sequencing. MZ01 could degrade crude oil by 54.7% within 9 days. The optimal conditions for MZ01 to produce biosurfactants were explored by orthogonal experiments. These conditions were yeast extract（3 g·L-1） as nitrogen source, corn oil（2 g·L-1） as carbon source, initial pH 9.0, 5% salinity and temperature 25 ℃. The production of biosurfactants by MZ01 was 2.27 g·L-1 after 3-day fermentation. The critical micelle concentration（CMC） of the biosurfactants was 0.1 g·L-1, reducing the surface tension of pure water from 72 mN·m-1 to 30 mN·m-1. |
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