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| Induction and Its Molecular Mechanisms of Chilling Tolerances of Early Rice Seedlings with Inorganic Salts and γ-Polyglutamic Acid |
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| KeyWord:early rice; chilling stress; γ-polyglutamic acid; seed treatment; RT-PCR |
| Author Name | Affiliation | | ZHANG Yan-fang | MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China | | ZHOU Hui-mei | MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China | | CAI Ke-tong | Xianning Academy of Agricultural Sciences of Hubei Province, Xianning 437000, Hubei , China | | ZHU An-ting | MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China | | XIE Guo-sheng | MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China |
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| Abstract: |
| Improving chilling tolerance of early rice is important for alleviation of chilling injury to early rice seedlings. Seeds of early rice Hua′ai 21, a chilling-sensitive variety, were first subjected to treatments for 36 h with CaCl2, inorganic salt mixture, salicylic acid（SA） and jasmonic acid（JA） in combination with γ-polyglutamic acid（PGA）, respectively. The seedlings of seven-day old were then grown under chilling（6 ℃） for 2 days followed by 25 ℃ for 3 days. Their growth and physiological characteristics were studied in hydroponic conditions. Seed treatments with different chemical compounds improved seedling tolerance to chilling stress, and increased soluble sugar contents and APX and SOD activities, but decreased MDA contents with the greatest effects observed in the treatment with inorganic salt mixture in combination with γ-PGA. Under chilling stress, the transcription level of OsICE1 gene did not change, whereas that of other 5 cold-responsive marker genes was enhanced. Inorganic salt mixture in combination of γ-PGA induced genes OsDREB1B and OsCDPK7 expression, but, inhibited OsLIP19 gene expression. It is concluded that seed treatment with inorganic salt mixture combined with γ-PGA could enhance the chilling tolerance of early rice seedlings. |
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