|
| Pollution Characteristics of Antibiotic Resistance Genes in Xizhijiang-Dongjiang River Basin, Guangdong Province, China |
| |
| View Full Text View/Add Comment Download reader |
| KeyWord:water environment; ARGs; real-time PCR |
| Author Name | Affiliation | | ZHANG Rui-quan | State Key Laboratory of Organic Geochemistry，Guangzhou Institute of Geochemistry，Chinese Academy of Sciences，Guangzhou 510640，China | | YING Guang-guo | State Key Laboratory of Organic Geochemistry，Guangzhou Institute of Geochemistry，Chinese Academy of Sciences，Guangzhou 510640，China | | DING Yong-zhen | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | SU Hao-chang | State Key Laboratory of Organic Geochemistry，Guangzhou Institute of Geochemistry，Chinese Academy of Sciences，Guangzhou 510640，China | | LIU You-sheng | State Key Laboratory of Organic Geochemistry，Guangzhou Institute of Geochemistry，Chinese Academy of Sciences，Guangzhou 510640，China |
|
| Hits: 3212 |
| Download times: 2113 |
| Abstract: |
| Contamination from antibiotic resistance genes（ARGs） has become an environmental issue all over the world. In this study, we sampled water and sediments during dry season at ten sites in the Xizhijiang-Dongjiang River Basin, Guangdong province. SYBR Green-based real-time quantitative PCR was used to assay the abundance of three representative sulfonamides resistance genes（sul1, sul2, and sul3） and two integrase genes（int1 and int2）. These five genes were all detected in both water and sediment samples, implying ARG contamination of water in the Xizhijiang-Dongjiang River basin. The absolute abundance and relative abundance of sul1 and sul2 were highest, suggesting that they both were predominant ARGs. A positive correlation（R2=0.64） was found between the relative abundance of the ARGs in water and that in the sediments. In both water and sediments, there was a positive correlation between relative abundance of sul1 and int1（R2>0.95, P=0.000<0.01）, sul1 and sul2（R2>0.8, P=0.002<0.01）, and sul2 and int1（R2>0.8, P=0.004<0.01）. From tributaries to the main stream, antibiotic resistance gene became enriched. Special attention should be paid to ARGs contamination in this region. |
|
|
|
