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| Effects of Different Glyphosate-tolerance Soybeans on the Diversity of Nitrogen-fixing Bacteria in Rhizosphere Soil |
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| KeyWord:transgenic soybean; rhizosphere soil; nitrogen-fixing bacteria; diversity; nifH; DGGE-cloning |
| Author Name | Affiliation | | ZHANG Qiu-yan | Agro-Environmental Protection Institute, Ministry of Agriculture, Open Fund of Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-environment and Agro-product Safety
College of Life Science, Shanxi Agricultural University | | ZHAO Jian-ning | Agro-Environmental Protection Institute, Ministry of Agriculture, Open Fund of Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-environment and Agro-product Safety | | LI Gang | Agro-Environmental Protection Institute, Ministry of Agriculture, Open Fund of Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-environment and Agro-product Safety | | WANG Li-juan | Agro-Environmental Protection Institute, Ministry of Agriculture, Open Fund of Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-environment and Agro-product Safety
College of Life Science and Technology, Inner Mongolia Normal University | | WANG Hui | Agro-Environmental Protection Institute, Ministry of Agriculture, Open Fund of Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-environment and Agro-product Safety | | CHANG Hong | College of Life Science, Shanxi Agricultural University | | YANG Dian-lin | Agro-Environmental Protection Institute, Ministry of Agriculture, Open Fund of Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-environment and Agro-product Safety |
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| Abstract: |
| Transgenic crops are grown on an increasingly large scale around the world, and accompanied by public concern about their potential ecological and environmental impacts. The persistence and interaction of proteins derived from transgenic crops in soil and their effects on soil microbial are essential information when investigating their effects within the soil ecosystem. The nitrogen-fixing bacterial diversity in the rhizosphere of glyphosate-tolerance transgenic soybean M88、GTS40-3-2、ZB and conventional non-transgenic soybean zhonghuang13 was analyzed under pot experiments by DGGE-cloning technique. Soil samples were collected in November 2011 at mature stage. The results showed that the diversity index and evenness index of soil nitrogen-fixing bacteria of transgenic soybean M88（CP4 epsps）and GTS40-3-2（CP4 epsps） were not significantly different from non-transgenic soybean zhonghuang13. The diversity index of soil nitrogen-fixing bacteria of transgenic soybean ZB（Bt cry1Ab+CP4 epsps） was significantly higher than local major soybean zhonghuang13, while the evenness index showed no significant difference. The results of cloning and sequencing results showed that the soil nitrogen-fixing bacteria in the rhizosphere of the four soybean cultivars mainly belonged to α-proteobacteria, β-proteobacteria, γ-proteobacteria, cyanobacteria and unknown. These results suggested that compared with soybean varieties, the transformation of the CP4 epsps gene into soybean didn′t cause significant effect on the nitrogen-fixing bacterial diversity in soybean rhizosphere. |
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