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| Effects of Zinc Oxide Nanoparticles on Enzyme Activities of Soil Microorganisms |
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| KeyWord:zinc oxide nanoparticles; soil microorganisms; Microbacterium sp.; enzyme activities; oxidative damage |
| Author Name | Affiliation | | HOU Zhen | Laboratory for Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing 100871, China | | CHEN Zhuo | Laboratory for Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing 100871, China | | SHEN Zhao-yi | Laboratory for Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing 100871, China | | LI Ting-ting | Laboratory for Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing 100871, China | | YANG Jun-jun | Laboratory for Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing 100871, China | | LU Xiao-xia | Laboratory for Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing 100871, China |
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| Abstract: |
| Zinc Oxide Nanoparticles（ZnO-NPs） are among the most common engineered nanoparticles and widely used in various products, having potential impacts on ecological environment. The purposes of this study were to elaborate the influence of ZnO-NPs on soil microbial enzyme activities and to explore the action mechanism as well as the dose-response relationship. Via microcosm studies, pure bacteria（Microbacterium sp.） cultivation system and soil cultivation system were set up, and the microbial activities of Fluorescein Diacetate Hydrolase（FDAH） under the exposure of various concentrations of ZnO-NPs were measured. In the pure bacteria cultivation system, the Dehydrogenase（DH） activities were also measured. The results showed that, compared to the control medium without ZnO-NPs, ZnO-NPs in the liquid medium at concentrations of 1 mg·L-1, 5 mg·L-1 and 10 mg·L-1 significantly inhibited the activities of FDAH and DH of Microbacterium sp.with the inhibition rates being 22.5%, 61.2%, 62.3% and 27.8%, 44.8%, 44.8%, respectively. ZnO-NPs attached to the membranes of Microbacterium sp. and some entered the interior of the bacteria, causing oxidative damage. At ZnO-NPs concentrations of 5 mg·g-1 and 10 mg·g-1 soil, the activities of FDAH were significantly lower than that in the control（P=0.007 and P=0.008）. At ZnO-NPs concentration of 1 mg·g-1 soil, the activity of FDAH was not significantly different than that in the control（P=0.149）. The inhibition of FDAH activity was well positively correlated with the concentration of ZnO-NPs. Over the exposure time, the inhibition of ZnO-NPs on FDAH activity decreased. |
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