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| Effect of Aluminum Stress on Fungal Community Diversity in Acidic Red Soils |
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| KeyWord:aluminum stress; acidic red soils; fungal community diversity; PCR-RFLP |
| Author Name | Affiliation | | HE Gen-he | School of Life Sciences, Jinggangshan University, Ji′an 343009, China | | WANG Xiao-dong | Jiangxi Academy of Forestry, Nanchang 330013, China | | LIU Qing | School of Life Sciences, Jinggangshan University, Ji′an 343009, China | | WU Ji-chun | Department of Hydrosciences, Nanjing University, Nanjing 210093, China |
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| Abstract: |
| Aluminum（Al） in acidic soils shows toxicity to soil microbes. In this study, we constructed fungal internal transcribed spacer（ITS） rDNAs clone library using the PCR-amplified restriction fragment length polymorphism（PCR-RFLP） method, and comparatively studied the responses of fungal community in forest soil and agriculture soil to Al stress. A total of six fungal clone communities was obtained from the tested soil samples. Clones of each library were randomly selected for PCR-RFLP analysis of ITS rDNA fragments. Totally 77 genotypes were identified by RFLP fingerprinting and sequenced. These 77 genotypes were then sequenced and their respective phylotypes were identified through the Basic Local Alignment Search Tool（BLAST） of the National Center for Biotechnology Information（NCBI） with similarity of 95%~100%. Sequence results revealed that 79.2%（61） of the clone sequences have the highest similarity with those of the Ascomycota, Basidiomycota and Glomeromycota, and 20.8%（16） have near similarity with unclassified fungi. Basidiomycota was predominant in both soils. The Simpson, Shannon and Chao1 indices all showed that Al addition reduced the richness and diversity of soil fungi. However, the diversity of fungal communities was lower in the forest soil than in the agriculture soil. Elevated Al increased the relative abundance of Ascomycota but decreased that of Basidiomycota, thus evolving into dominant population in high Al soils. |
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