铜胁迫和间作对玉米抗氧化酶活性及丙二醛含量的影响

王晓维; 黄国勤; 徐健程; 聂亚平; 万进荣; 杨潇一; 杨文亭

Effects of Copper Stresses and Intercropping on Antioxidant Enzyme Activities and Malondialdehyde Contents in Maize

Received:April 23, 2014

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KeyWord:copper;antioxidases;malondialdehyde;intercropping

Author Name	Affiliation	E-mail
WANG Xiao-wei	Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
HUANG Guo-qin	Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
XU Jian-cheng	Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
NIE Ya-ping	Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
WAN Jin-rong	Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
YANG Xiao-yi	Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
YANG Wen-ting	Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China	ywt111@163.com

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Abstract:

With industrial development, the area of arable land contaminated by heavy metals is steadily increasing around the world, especially in developing countries. Intercropping has shown the potential to phytoremediate heavy metal polluted soils. A pot experiment was conducted to explore the antioxidant responses of maize to copper(Cu) pollution in red soil under maize-pea intercropping. Five Cu concentrations(0, 100, 200, 400, 600 mg·kg^-1) and two cropping patterns(maize monoculture and maize-pea intercropping) were designed. Plant biomass, Cu contents, antioxidant enzyme activities(SOD, CAT, POD) and MDA contents of maize were measured. Compared with those in the monoculture, the aboveground and underground dry biomass of maize in high Cu²⁺ concentration(600 mg·kg^-1) were increased by 20% and 36.6%, respectively, under the intercropping system. However, Cu contents in the aboveground were reduced by 86.81%, 44.57%, 22.01% and 86.81%, but root Cu increased by 78.89%, 24.79%, 35.29% and 24.79% in 100, 200, 400 mg·kg^-1 and 600 mg·kg^-1 treatments, respectively, under the intercropping system, compared with the maize monoculture. Significant difference in plant Cu was present between intercropping and monoculture. The SOD, POD, CAT activities and MDA content in leaves and roots of maize all increased with increasing Cu²⁺ concentrations. But Cu²⁺ addition at 600 mg·kg^-1 caused CAT activity decrease. Compared to the monoculture, SOD activity under maize-pea intercropping increased by 48.07%~117.27% in roots and 11.30%~46.90% in leaves. Cropping patterns had no significant effect on POD activity under the same Cu²⁺ stress. In 0~400 mg·kg^-1 Cu stresses, CAT activities in maize leaves were significantly increased by 71.37%, 140.40%, 229.80% and 161.75% respectively under the intercropping over the monoculture, whereas no significant difference in the CAT activities in roots was observed between two cropping systems. The intercropping practice reduced MDA contents in maize roots by 26.13%~64.53%; while intercropping caused left MDA decline only at 100 mg Cu·kg^-1 and 200 mg Cu·kg^-1 but increased at 400 mg Cu·kg^-1 and 600 mg Cu·kg^-1, as compared with the monoculture. In summary, intercropping could improve antioxidant enzymatic system in maize roots and leaves and thus alleviate Cu toxicity under Cu²⁺ stresses.