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| Acute Toxicity of Technical Material and Different Formulations of Difenoconazole and Azoxystrobin to Zebrafish |
| Received:April 19, 2014 |
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| KeyWord:difenoconazole;azoxystrobin;formulation;zebrafish;acute toxicity |
| Author Name | Affiliation | E-mail | | ZHANG Guo-fu | College of Plant Protection, Research Center of Pesticide Environmental Toxicology, Key Laboratory of Pesticide Toxicology & Application Technique, Shandong Agricultural University, Taian 271018, China | | | LI Ben-jie | College of Plant Protection, Research Center of Pesticide Environmental Toxicology, Key Laboratory of Pesticide Toxicology & Application Technique, Shandong Agricultural University, Taian 271018, China | | | WANG Jin-hua | College of Resources and Environment, Shandong Agricultural University, Taian 271018, China | | | XIA Xiao-ming | College of Plant Protection, Research Center of Pesticide Environmental Toxicology, Key Laboratory of Pesticide Toxicology & Application Technique, Shandong Agricultural University, Taian 271018, China | xxm@sdau.edu.cn |
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| Abstract: |
| Different formulations of pesticides show various toxicities to organisms. In this study, an acute toxicity experiment was conducted to evaluate the ecological risks of technical material and different formulations of difenoconazole and azoxystrobin to adult zebrafish using semi-static test. The 96 h LC50 values and 95% confidence limits for 95% difenoconazole technical material(TC), 3% difenoconazole flowable concentrate for seed coating(FS), 400 g·L-1 difenoconazole aqueous suspension concentrate(SC), and 60% difenoconazole water dispersible granule(WG) for adult zebrafish were 1.05 and 0.93~1.13, 1.34 and 1.26~1.39, 1.44 and 1.37~1.52, and 2.72 and 2.68~2.76 a.i.mg·L-1, respectively. Those of 93% azoxystrobin technical material(TC), 10% azoxystrobin flowable concentrate for seed coating(FS), 25% azoxystrobin aqueous suspension concentrate(SC), and 50% azoxystrobin water dispersible granule(WG) for adult zebrafish were respectively 0.67 and 0.64~0.72, 0.88 and 0.85~0.92, 1.03 and 0.98~1.07, and 1.60 and 1.10~1.81 a.i.mg·L-1. According to the Experimental Guideline for Environmental Safety Evaluation of Chemical Pesticides, the toxicity of TC and different formulations of difenoconazole and that of 25% azoxystrobin SC and 50% azoxystrobin WG to adult zebrafish were moderate, while that of 93% azoxystrobin TC and 10% azoxystrobin FS was high. These results indicate that the toxicity of TC and different formulations of difenoconazole and azoxystrobin to adult zebrafish is different, being TC > FS > SC > WG. |
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