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| Effect of Carbon Source Concentrations on Microbial Hydrogen Production and Fe(Ⅲ) Reduction |
| Received:November 22, 2014 |
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| KeyWord:glucose concentration;fermentative hydrogen production;Fe(Ⅲ) reduction;electron consumption ratio |
| Author Name | Affiliation | E-mail | | YOU Ping | College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China | | | JIA Rong | College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China | | | QIAO Sha-sha | College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China | | | QU Dong | College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China | dongqu@nwafu.edu.cn |
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| Abstract: |
| Fermentative Fe(Ⅲ) reducing microorganism as a facultative anaerobe can reduce Fe(Ⅲ) to Fe(Ⅱ) by using the intermediates of glucose metabolism as an electron donor. However, the effects of carbon source concentrations on microbial hydrogen production and Fe(Ⅲ) reduction were unclear. A 30-d anaerobic pure incubation experiment with Fe(Ⅲ) reducing bacteria JX1-25 was conducted to investigate the effects of glucose concentrations on Fe(Ⅲ) reduction, pH and hydrogen partial pressure. When glucose was the sole carbon source, Fe(Ⅲ) reduction rates by the strain JX1-25 were 6.81%, 12.47%, 56.69%, 97.71%, 96.86% and 99.77% for 0.25, 0.5, 2, 4, 8 mmol·L-1 and 16 mmol·L-1 glucose, respectively. The Fe(Ⅲ) reduction potential(a) and maximum reduction rate(Vmax) and hydrogen pressure peak(ppH2max) increased with increasing glucose concentrations. Electron consumption percentages of microbial Fe(Ⅲ) reduction and hydrogen production were respectively 2.02%~9.69% and 13.74%~19.45% at different concentrations of glucose. Glucose at 4 mmol·L-1 was able to satisfy the demand of Fe(Ⅲ) reduction. Canonical correspondence analysis(CCA) indicated that there was to some extent correlation between microbial Fe(Ⅲ) reduction and hydrogen production, in which the strain JX1-25 used glucose as carbon source. The peak of hydrogen partial pressure(ppH2max) had an extremely significant positive correlation with a and Vmax but a negative correlation with the time to the maximum reduction rate(TVmax) and the minimum pH value of the incubation system(pHmin). |
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