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| Leaching of CuO Nanoparticles with Copper Tolerant Bacteria and Their Communities |
| Received:January 20, 2015 |
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| KeyWord:heavy metal;acclimation;leaching |
| Author Name | Affiliation | E-mail | | ZAN Shu-ting | College of Environmental Science and Engineering, Anhui Normal University, Wuhu 241003, China | | | ZHOU Gang | College of Environmental Science and Engineering, Anhui Normal University, Wuhu 241003, China | | | LI Jing | College of Environmental Science and Engineering, Anhui Normal University, Wuhu 241003, China | | | SU Nan-nan | College of Environmental Science and Engineering, Anhui Normal University, Wuhu 241003, China | | | SHAO Zong-yuan | College of Environmental Science and Engineering, Anhui Normal University, Wuhu 241003, China | | | YANG Ru-yi | College of Environmental Science and Engineering, Anhui Normal University, Wuhu 241003, China | yangruyi@mail.ahnu.edu.cn |
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| Abstract: |
| Behavior, environmental fate and biotoxicity of manufactured nano materials(MNMs) have received extensive attention in recent years. However, it is unclear whether MNMs-contaminated soils could be remediated by combined plant and rhizospheric microbes. In this study, bacterial community structure associated with copper(Cu) accumulating plant Artemisia capillaris Thunb. was investigated using PCR-DGGE and sequencing techniques. Subsequently, Cu tolerant bacteria were screened using selective culture medium containing 500 mg·L-1 CuSO4. The effect of Cu tolerant bacteria and bacterial communities on leaching of nano CuO(CuO NPs) was then studied. Results showed that bacterial community in the rhizosphere had very low diversity, and mainly consisted of genus of Aerococcus, Stenotrophomonas and Micrococcus, with Aerococcus being the dominant genus. The growth of bacterium S31 was enhanced by low CuO NPs, but suppressed by 400 mg·L-1 CuO NPs. The Cu tolerant bacterium S31 showed a concentration dependent enhancement on leaching of CuO NPs. Acclimation significantly improved the growth of bacterium S31 and increased the leaching of CuO NPs. This study indicates the potential to remediate MNMs-contaminated soil by combined plant and microbes. |
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