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Pepper Growth Influenced by and Formula Optimization and Mechanisms of Remediation for DBP/DIBP
Received:March 03, 2015  
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KeyWord:attapulgite;pepper;dibutyl phthalate(DBP);diisobutyl phthalate(DIBP)
Author NameAffiliation
REN Xu-qin Faculty of Life Science & Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, China
Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huaian 223003, China 
GAO Jun Faculty of Life Science & Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, China
Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huaian 223003, China 
CHEN Bo-qing Faculty of Life Science & Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, China
Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huaian 223003, China 
LUO Dan-dan Faculty of Life Science & Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, China 
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Abstract:
      In the present study, the effects of dibutyl phthalate(DBP) and diisobutyl phthalate(DIBP) on pepper growth were studied, and the remediation formula for DBP/DIBP was also optimized using Box-Behnken experimental design and regression model. Meanwhile, the remediation mechanisms were discussed. The growth and dry weight of pepper were significantly inhibited by 20 mg·kg-1 DBP/DIBP in soil. The regression model predicted the remediation effects of formula applied very well(P<0.01). The optimized formula was 15.91 g·kg-1 of attapulgite, 16.40 g·kg-1 of honeycomb residue and 1.67 g·kg-1 of humic acid. Applying optimized formula significantly increased urease, dehydrogenase, protease and saccharase activities in rhizospheric soil, and improved peroxidase(POD) and catalase(CAT) in pepper roots, but decreased MDA content as compared to the control. Under optimized formula, the area and activity of pepper roots were increased and the dry matter of pepper improved by 20.91%. The application of optimized formula effectively alleviated the negative effects of DBP/DIBP on pepper.