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| Screening and Identification of Cellulose-Degrading Bacteria from Spent Substrate of Edible Mushroom |
| Received:March 06, 2015 |
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| KeyWord:spent substrate;cellulose degrading microorganisms;screening;16S rDNA sequence;enzymatic activity;complex bacteria;compost |
| Author Name | Affiliation | E-mail | | LIU Xiao-mei | Institute of Agricultural Resources and Regional Planning, Center for Quality Supervision and Test of Microbial Fertilizers and Mushroom Spawn, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China | | | ZOU Ya-jie | Institute of Agricultural Resources and Regional Planning, Center for Quality Supervision and Test of Microbial Fertilizers and Mushroom Spawn, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China | | | HU Qing-xiu | Institute of Agricultural Resources and Regional Planning, Center for Quality Supervision and Test of Microbial Fertilizers and Mushroom Spawn, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China | huqingxiu@caas.cn | | YANG Xiao-hong | Institute of Agricultural Resources and Regional Planning, Center for Quality Supervision and Test of Microbial Fertilizers and Mushroom Spawn, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China | | | SHEN De-long | Institute of Agricultural Resources and Regional Planning, Center for Quality Supervision and Test of Microbial Fertilizers and Mushroom Spawn, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China | shendelong@caas.cn |
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| Abstract: |
| The cellulose in spent substrate of edible mushroom is often a limiting factor of the spent substrate decomposition. This study was performed to isolate highly effective cellulose-degrading bacteria from spent substrate in order to improve the fermentation of spent substrate of edible mushroom. Samples were collected from spent substrates of Pleurotus eryngii at different stacking places. Four strains(FB7, CB1, BC11 and BC12) with high cellulose-degrading efficiencies were obtained based on the results of Congo red cellulose hydrolytic hole measurement, filter paper degradation test and cellulose activity assays(filter paper enzyme FPA, endoglucanase CMCase, exoglucanase C1 and glucosidase β-Gase), using CMC-Na as sole carbon source. 16S rDNA sequencing showed that the trains FB7 and CB1 were Bacillus subtilis, the strain BC11 was Streptomyces albus, and the strain BC12 was Comamonas_ jiangduensis. The strain FB7 with high filter paper degrading ability had high cellulose activities and degraded filter paper into paste within 10 days. The activities of four enzymes (FPA, CMCase, C1 and β-Gase) produced by the stain FB7 cultured for 4 days on a shaking incubator were 22.81 U·g-1, 314.50 U·g-1, 2.78 U·g-1 and 188.09 U·g-1, respectively. The FPA, CMCase, C1, and β-Gase activities of bacterial combination(CB1+BC11+FB7) were 31.56 U·g-1, 133.61 U·g-1, 2.31 U·g-1, and 217.21 U·g-1, increased by 38.4%, 11.2%, 178%, and 70.3%, respectively, compared with the strain FB7 alone. Inoculation of bacterial combination(CB1+BC11+FB7) to spent substrate compost increased the temperature of the compost quickly, with higher and lesser fluctuations than in the control. |
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