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| Abundance and diversity of sulfate-reducing bacteria in littoral wetland of Wuliangsuhai |
| Received:September 18, 2015 |
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| KeyWord:littoral wetland;sulfate-reducing bacteria(SRB);community structure;functional gene apsA |
| Author Name | Affiliation | E-mail | | LIU Jian-li | College of Environment & Resources of Inner Mongolia University, Huhhot 010021, China | | | ZHAO Ji | College of Environment & Resources of Inner Mongolia University, Huhhot 010021, China | | | WU Lin-hui | College of Environment & Resources of Inner Mongolia University, Huhhot 010021, China | happyleis@sina.com |
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| Abstract: |
| Abundance and diversity of sulfate-reducing bacteria(SRB) in sediments and soils from landward transect of Wuliangsuhai were investigated using clone library construction and real-time quantitative PCR(q-PCR) techniques. The abundance of apsA gene was significantly different among four studied samples. It was 1.34×107, 8.07×105, 5.27×106, and 1.34×107 copies per gram of sample for landward evolved Phragmites australis sediments, lakeside Scirpus validus Vah sediments, lakeside Suaeda salsa salinized meadow soil, and lakeshore desertificated Nitraria tangutorum soil, respectively. Phylogenetic tree analysis showed that SRB communities in Wuliangsuhai consisted of five families. They were Acidithiobacillales, Desulfurella, Chromatiales, Desulfovibrionaceae and Desulfobacteriaceae in the Proteobacteria phylum. Among them, Chromatiales, Desulfovibrionaceae and Desulfobacteriaceae were dominant. The diversity of SRB was closely correlated with total water soluble salt, moisture content, and sulfate content(P<0.05). |
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