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| Distribution of protein molecular in seedlings of Lycium barbarum and Brassica juncea under vanadiumstresses |
| Received:November 15, 2015 |
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| KeyWord:vanadium stress;Lycium barbarum;Brassica juncea;molecular distribution;protein |
| Author Name | Affiliation | | HOU Ming | College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China | | ZHAO Jun-ping | College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China | | LI Ming-yuan | College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China | | GAN Huan-hui | College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China |
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| Abstract: |
| Protein molecular distribution in Lycium barbarum and Brassica juncea under different vanadium(V) stress levels was studied by G-75 gel chromatography and electrophoresis analysis. After gel chromatography, two UV peaks appeared, with the first peak occurring in No.8~No.15 tubes, and the second peak in No.25~No.33 tubes. Results of electrophoresis analysis from the first UV peak indicated that the molecular weight distribution changed under the V stresses. At 10 mg V·L-1, Lycium barbarum produced a kind of protein with molecular weight of 58.6 KD in roots, while in stems two kinds of protein with molecular weight of 64.5 and 46.9 KD. At 20 mg V·L-1, the leaves produced a kind of protein with molecular weight of 20 KD. With V stress levels increasing, two protein bands with 33.9 KD and 36 KD in Brassica juncea gradually changed to shallow, but that with 27.3 KD was deepened progressively. The V concentrations in the protein of the first elution peak in Lycium barbarum seedlings increased with increasing V stress levels, while the V concentrations in leaves of Brassica juncea decreased when V stress levels ≥10 mg·L-1. This may be due to differences in V-binding capacity of different plant proteins. The V-binding ability of Lycium barbarum seedlings was greater than that of Brassica juncea, and thus more phytochelatins were generated, resulting in stronger tolerance of Lycium barbarum seedlings to V. |
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