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| Effects of indole-3-acetic acid on morphologic and physiological characteristics of root systems of plants with different arsenic-accumulating abilities under As stress |
| Received:December 16, 2015 |
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| KeyWord:indole-3-acetic acid;hyperaccumulator;arsenic;root system;root activity |
| Author Name | Affiliation | E-mail | | HE Shu-juan | Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China | | | WANG Hong-bin | Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China | whb1974@126.com | | WANG Hai-juan | Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China | | | ZHAO Bin | Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China | | | LI Qin-chun | Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China | |
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| Abstract: |
| A hydroponic experiment was conducted to investigate the effects of indole-3-acetic acid(IAA) on growth, root morphology, root activity, root ATPase activity in cytoplasmic membrane, root antioxidative enzymes(superoxide dismutase SOD, catalase CAT and proxidase POD), malondialdehyde(MDA, a peroxidation product of membrane lipids) content, and As accumulation in plants with different As-accumulating abilities. Arsenic hyperaccumulator(Pteris cretica var. nervosa) and non-hyperaccumulator(Pteris ensiformis) were exposed to 2 mg·L-1 As(V) and different concentrations of IAA(0,10,20,40,and 60 mg·L-1) for 14 d. Results showed that biomass of two plants significantly increased in the presence of 20 mg·L-1 IAA, compared with the control. At the 20 mg·L-1 IAA treatment, As concentrations in fronds and petioles, root activity, and root ATPase activity in cytoplasmic membrane of P. cretica var. nervosa increased significantly, and were significantly higher than those in P. ensiformis. At 20~60 mg·L-1 IAA treatments, the activities of three antioxidative enzymes(CAT, SOD and POD) in roots of P. cretica var. nervosa increased significantly, but the CAT activity decreased and SOD activities increased significantly in roots of P. ensiformis, in comparison with no IAA treatment. The POD activity showed no significant changes in the roots of P. ensiformis. The content of MDA in roots of both plants were significantly decreased by 20~40 mg·L-1 IAA treatments. However, MDA content in the roots of P. cretica var. nervosa was significantly lower than that of P. ensiformis. Correlation analysis showed that As concentrations in roots of P. cretica var. nervosa were positively related with root activity, whereas no correlation was found between root As concentrations and root parameters in P. ensiformis. Therefore, As hyperaccumulation of P. cretica var. nervosa was attributed to its high root activities. |
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