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| The distribution and toxic effects of Pb at the levels of the tissue and sub-cellular in Neyraudia reynaudiana |
| Received:May 06, 2016 |
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| KeyWord:Pb, Neyraudia reynaudiana, tissue, sub-cellular, scanning electron microscope(SEM), transmission electron microscope with energy dispersive x-ray spectroscopy(TEM-EDS) |
| Author Name | Affiliation | E-mail | | HUANG Mei-ying | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Co-innovation Center for Soil and Water Conservation in Red Soil Region of the Cross-straits, Fuzhou 350002, China | | | LUO Jie-wen | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Co-innovation Center for Soil and Water Conservation in Red Soil Region of the Cross-straits, Fuzhou 350002, China | | | HUANG Cai-feng | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Co-innovation Center for Soil and Water Conservation in Red Soil Region of the Cross-straits, Fuzhou 350002, China | | | ZHOU Chui-fan | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Co-innovation Center for Soil and Water Conservation in Red Soil Region of the Cross-straits, Fuzhou 350002, China | zhouchuifan@163.com |
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| Abstract: |
| In order to examine the phytoremediation potential of Neyraudia reynaudiana,the study explored the toxic effects and distribution of lead(Pb) at the levels of the tissue and sub-cellular in this species and analyzed the supplementing the growth medium with Pb as a target pollutant. After cultivation was concluded,the uptake of sub-cellular Pb was determined,and the distribution of Pb as well as the Pb-induced damage was respectively assessed through scanning electron microscope(SEM) and transmission electron microscope with energy dispersive X-ray spectroscopy(TEM-EDS). Pb caused the tissues to become irregular and fragmented by injuries;additionally,a portion of the catheters were filled with crystals.At the sub-cellular level,many phenomena demonstrated that leaf and root cells were harmed;for example,cell walls became vague,mitochondria were less numerous,and chloroplasts were swollen. Pb was mainly distributed in the cell wall(12.28 mg·kg-1) and the vacuole(38.82 mg·kg-1) of root cells,and it accounted for 32.56% of the total biomass in the leaves.In addition, the EDS analysis found a significant amount of Pb in cells.These results suggest that N.reynaudiana has a strong ability to both absorb and tolerate Pb. Moreover,the species may alter the distribution of Pb at both the tissue and the sub-cellular levels to reduce toxicity.Therefore,N.reynaudiana is well suited for phytoremediation in Southern China and elsewhere. |
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