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| The distribution characteristics and potential risk of tetracycline, oxytetracycline and their corresponding genes pollution in sediment of Hongze Lake |
| Received:September 24, 2016 |
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| KeyWord:Hongze Lake;tetracycline antibiotics;ARGs;HPLC-MS;qPCR |
| Author Name | Affiliation | E-mail | | LUO Fang-yuan | College of Resourse and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China
Nanjing Institute of Environmental Science, Ministry of Environmental Protection of the People's Republic of China, Nanjing 210042, China | | | PAN Gen-xing | College of Resourse and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China | | | LI Lian-qing | College of Resourse and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China | | | ZHANG Jun | Nanjing Institute of Environmental Science, Ministry of Environmental Protection of the People's Republic of China, Nanjing 210042, China | | | WANG Na | Nanjing Institute of Environmental Science, Ministry of Environmental Protection of the People's Republic of China, Nanjing 210042, China | | | JIAO Shao-jun | Nanjing Institute of Environmental Science, Ministry of Environmental Protection of the People's Republic of China, Nanjing 210042, China | xuhuizhang@njau.edu.cn | | ZHANG Xu-hui | College of Resourse and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China | |
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| Abstract: |
| Antibiotic resistance genes(ARGs) have been a great concern to the environmental health in the world. This study was focus on the content, distribution and potential risk of tetracycline, oxytetracycline and the corresponding resistance genes in the sediments from the Hongze Lake, to find out the relationship between the selected antibiotics and its induced resistance genes. The sediment samples was collected in forty two spots all over the lake for the subsequent analysis. The high-performance liquid chromatography-mass spectrograph(HPLC-MS) was used to investigate the concentration of two common tetracycline antibiotics(tetracycline, oxytetracycline), moreover, quantitative real-time PCR was used to study the abundance of three typical tetracycline resistance genes in the samples. The results showed that the two tetracycline(TCs) antibiotics are detected in all samples, with the contents of tetracycline antibiotics ranging from 1.35 μg·kg-1 to 25.43 μg·kg-1. The tetA and tetC genes were detected in all samples, in contrast, the tetM gene was partially found in some samples. Among the three genes, tetC gene was in a higher level than other two genes. Furthermore, the relevance analysis indicated that the abundance of tetracycline resistance genes in the sediment of Hongze Lake, were not only related to content of tetracycline antibiotics, but also to the other environmental factors as organic carbon. This result showed that the appearance of ARGs in the environment is a comprehensive process, and the elimination of ARGs should be based on the well treatment on the antibiotics as well as other ambient factors. |
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