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| Construction of petroleum degrading bacteria consortium and its degradation properties of mixed hydrocarbons |
| Received:September 30, 2016 |
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| KeyWord:petroleum contaminated soil;optimal condition;bioremediation;microbial community |
| Author Name | Affiliation | | FAN Rui-juan | College of Biological Science & Engineering, Beifang University of Nationalities, Yinchuan 750021, China | | GUO Shu-hai | Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | | LI Feng-mei | Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China |
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| Abstract: |
| After enrichment, separation, purification, screening by selective medium, and testing by degradation ability of the total petroleum hydrocarbon(TPH), 6 strains(B1, B2, B3, B6, B7 and B9) with cyclanes, alkanes, and aromatics degradation ability were obtained. Based on the sequence analysis of 16S rRNA gene, B1, B2, B6 and B7 were identified as Hydrogenophaga sp., Phenylobacterium sp., Sphingobium sp. and Bacillus sp., respectively; B3 and B9 were identified as Arthrobacter sp.. A microbial degradation flora was constructed through the optimization of the inoculum proportion, size, and growth conditions. The degradation flora was then used in the remediation of mixed hydrocarbon-contaminated soil, the results demonstrated an obvious effect of the flora in the degradation of three kinds of hydrocarbons. After 40 d of remediation, the degradation extent of cyclododecane can be reach to 74.5%; and after 100 d, the degradation extents of n-hexadecane and pyrene reached to 56.9% and 60.4%, respectively. There was a significant decrease of pollutants during the first 10 d, and the removal efficiency was reduced along with the experiment, showed a similar variation tendency with microbial diversity, quantity and activity in soil. |
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