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| Analysis of Cd-induced DNA damage in Arabidopsis seedlings |
| Received:November 21, 2016 |
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| KeyWord:Cd;DNA damage;DNA repair;cell cycle |
| Author Name | Affiliation | E-mail | | SONG Jie | School of Environmental Science, Liaoning University, Shenyang 110036, China
Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | | | WANG He-tong | Liaoning He University, Shenyang 110163, China | | | CUI Wei-na | Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
Shanghai Institute of Technology, Shanghai 201418, China | | | SUN Li-zong | Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | | | CAO Xia | Shenyang Agricultural University, Shenyang 110866, China
Institute of Vegetable Science, Tongliao Academy of Agricultural Sciences, Tongliao 028000, China | | | HE Lei | Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | | | JIANG Li-si | Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | | | CHENG Zhi-bo | Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | | | HUI Xiu-juan | School of Environmental Science, Liaoning University, Shenyang 110036, China | | | TAI Pei-dong | Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | | | YANG Yue-suo | Laboratory of Ecological Restoration of Polluted Environment and Resource Technology, Shenyang University, Shenyang 110044, China | | | LIU Wan | Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | liuwan63@hotmail.com |
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| Abstract: |
| DNA damage assay based on RAPD, ELISA-based global methylation analysis, and expression analysis of genes related to DNA damage, repair, and cell cycle were used to study genomic DNA damage and stress response of DNA repair and cell cycle in Arabidopsis plantlets exposed to 0,0.125,0.25,1.0 mg·L-1 and 2.5 mg·L-1 cadmium(Cd) for 5 d. Compared with the control, DNA damage and global methylation rate were increased significantly with the increased Cd dose, and expression of cell division genes(PCNA1 and PCNA2), MMR genes(MLH1, MSH2 and MSH6), homologous recombination genes(RAD51 and BRCA1), and non-homologous end joining genes(KU70, MRE11 and GR1) were followed an obvious inverted U-shaped dose-response effect of Cd exposures and a MMR > HR > NHEJ sensitivity rule. The results showed that low-dose Cd result in easy-repaired mismatch damage, and high-dose Cd stress cause difficult-repaired chromosome damage and double strand break, which could accumulate and aggravate with the stress duration. In addition, the expression of MSH6 and MLH1 is the most sensitive indicator which could be a sensitive biomarker, applied to early diagnosis and risk assessment of genotoxic effects of Cd pollution in ecotoxicology. |
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