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| Comparison and physiological mechanisms of cadmium (Cd) accumulation by strain J1 and mutant J77 of Agaricus brasiliensis |
| Received:January 13, 2017 |
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| KeyWord:Agaricus brasiliensis;cadmium (Cd);accumulation;heavy metal |
| Author Name | Affiliation | E-mail | | LIU Peng-hu | National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China | | | LI Bo | College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China | | | JIANG Zhi-he | Institute of Soil and Fertilizer Sciences, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China | | | WANG Yi-xiang | Institute of Agricultural Ecology, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China | | | WENG Bo-qi | Institute of Agricultural Ecology, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China | wengboqi@163.com |
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| Abstract: |
| Agaricus brasiliensis is an edible and medicinal mushroom with a strong capability of cadmium accumulation, which may impact food security. Using a combination of 60Co-γ ray and UV light, we previously bred a new A. brasiliensis strain named J77 by irradiating mycelium of strain J1. Owing to its low Cd content and high stable yield, J77 was approved as a new variety named Fuji 77 by the Crop Variety Approval Committee of Fujian Province in 2013. Using the A. brasiliensis strain J1 and its mutant J77 as research materials, the effects of Cd in different concentrations on mycelium growth rate and yields, Cd accumulation and contents of three amino acids in mycelium, and fruiting bodies of J1 and J77 were analyzed by adding different concentrations of Cd into the culture medium to reveal the differences in Cd uptake, tolerance, and physiological response mechanisms between J1 and J77. The results showed that mycelium growth rate and yields of J1 and J77 increased first and then declined with the increase of Cd concentration, which indicated that Cd of lower concentrations could promote the growth of mycelium and increase the yields. Under the same concentration of Cd, Cd contents in both mycelium and fruiting body of J77 were significantly lower than those of J1, which indicated that J77 was a lower Cd accumulative strain. In addition, there was a significant positive correlation between Cd contents in mycelium and fruiting bodies (R2>0.97). Cysteine (Cys) contents in both fruiting bodies of J1 and J77 decreased first and then increased with the increase of Cd concentrations. Cys content was significantly higher in fruiting body of J1 compared with J77 under lower Cd concentrations. In contrast, the Cys content in the fruit body of J77 was significantly higher than that of J1 under higher Cd concentrations. These results indicated that Cys might play an important role in Cd accumulation in A. brasiliensis. |
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