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| Effects of Pb stress on physiological characteristics and subcellular distribution of Pb in Isachne globosa |
| Received:December 09, 2016 |
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| KeyWord:Pb hyperaccumulator;Isachne globosa;organic acid;non-protein thiols;cell compartmentation |
| Author Name | Affiliation | E-mail | | CHEN Shun-yu | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Co-innovation Center for Soil and Water Conservation in Red Soil Region of the Cross-straits, Fuzhou 350002, China | | | HAN Hang | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Co-innovation Center for Soil and Water Conservation in Red Soil Region of the Cross-straits, Fuzhou 350002, China | | | CHEN Jia-song | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Co-innovation Center for Soil and Water Conservation in Red Soil Region of the Cross-straits, Fuzhou 350002, China | | | CAI Li-ping | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Co-innovation Center for Soil and Water Conservation in Red Soil Region of the Cross-straits, Fuzhou 350002, China | | | HOU Xiao-long | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Co-innovation Center for Soil and Water Conservation in Red Soil Region of the Cross-straits, Fuzhou 350002, China
College of Resource and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China | xl.hou@fafu.edu.cn | | LIU Ai-qin | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Co-innovation Center for Soil and Water Conservation in Red Soil Region of the Cross-straits, Fuzhou 350002, China | | | ZHOU Chui-fan | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Co-innovation Center for Soil and Water Conservation in Red Soil Region of the Cross-straits, Fuzhou 350002, China | |
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| Abstract: |
| Isachne globosa, a type of Pb hyperaccumulator, was selected as a study subject. An indoor hydroponic Pb stress experiment was designed and five different Pb concentrations (0, 250, 500, 1000, and 1500 mg·L-1) were set to identify the concentrations of the different chemical forms of Pb, organic acids, and non-protein thiols (NPT) and the distribution of Pb, and then to reveal the response mechanism of Isachne globosa to high Pb stress. The results showed that with the increase in Pb concentration, the concentrations of Pb of different chemical forms in the shoot and root of Isachne globosa were significantly increased (P<0.05). Pb primarily existed in roots and leaves of Isachne globosa as HCl-extractable forms, secondly as HAc-and NaCl-extractable forms, and was barely found as H2O-extractable and residual forms. In addition, the citric acid, malic acid, and NPT concentrations of Isachne globosa significantly increased (P<0.05) along with the increase in Pb concentration. Observations through an electron microscope revealed that the most of Pb existed in the cell walls of the root and shoot of Isachne globosa and an obvious cell compartmentation was detected. We therefore concluded that the chelation of organic acids and NPT and the cell compartmentation of Pb played important roles in the adaptation of Isachne globosa to the high Pb stress environment. |
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