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| Alleviation of cadmium-induced oxidative stress in Sinopotamon henanense ovarian tissue by N-acetylcysteine |
| Received:December 02, 2016 |
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| KeyWord:Sinopotamon henanense;ovary;N-acetylcysteine(NAC);oxidative damage;cadmium |
| Author Name | Affiliation | E-mail | | CHEN Hong-miao | School of Life Science, Shanxi University, Taiyuan 030006, China | | | XU Peng | School of Life Science, Shanxi University, Taiyuan 030006, China | | | WU Zhong-qiang | School of Life Science, Shanxi University, Taiyuan 030006, China | | | JING Wei-xin | School of Life Science, Shanxi University, Taiyuan 030006, China | | | WANG Lan | School of Life Science, Shanxi University, Taiyuan 030006, China | lanwang@sxu.edu.cn |
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| Abstract: |
| The aim of this study was to investigate the effects of N-acetylcysteine(NAC) on alleviation of cadmium-induced oxidative stress in Sinopotamon henanense ovarian tissue using the following six indicators:the tissue accumulation of Cd; the activities of antioxidant enzymes superoxide dismutase(SOD), catalase(CAT), and glutathione peroxidase(GPx); the content of malonyldialdehyde(MDA); and the changes in the microstructure of ovarian tissue. The experiment included three groups:the control group, the Cd2+(0.725 mg·L-1) group, and the group of Cd2+(0.725 mg·L-1) combined with NAC at three different levels of concentration(0.1, 0.2, or 0.4 g·L-1). The results showed that under the action of cadmium alone, with increasing Cd2+ concentration the accumulation of Cd and the content of MDA significantly increased as compared to that in the control group. The activities of SOD, CAT, and GPx increased at different time-points. The microstructure of ovarian tissue was damaged at different levels. For the group of Cd2+ combined with NAC at three different levels of concentration, the content of Cd in ovarian tissue decreased significantly on the 14th and the 28th days. The activity of SOD decreased significantly on the 14th day. The activities of CAT, GPx, and the content of MDA had a significant decrease on the 28th day, and returned to normal levels gradually. With the extension of time and the increase in NAC concentration, the damage to ovarian tissues was alleviated. That is, nuclear membranes were gradually repaired, and there was only a small quantity of chromatin agglutinate. The vacuolation in cells and nuclei almost disappeared, with the structure of ovarian cells returning to normal conditions. The results indicated that NAC could alleviate the cadmium-induced reproductive toxicity in Sinopotamon henanense. The results of our study support the argument that oxidative stress is one of the important underlying mechanisms of reproductive toxicity of cadmium. |
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