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| Hydrogen sulfide regulates cadmium stress resistance through P-ATPases in foxtail millet seedlings |
| Received:June 27, 2017 |
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| KeyWord:foxtail millet seedlings;hydrogen sulfide;cadmium stress;adenosine triphosphate |
| Author Name | Affiliation | E-mail | | LI Juan | College of Life Science, Shanxi University, Taiyuan 030006, China | | | TIAN Bao-hua | Taiyuan University of Technology, Taiyuan 030024, China | | | LIU Dan-mei | College of Life Science, Shanxi University, Taiyuan 030006, China | | | PEI Yan-xi | College of Life Science, Shanxi University, Taiyuan 030006, China | peiyanxi@sxu.edu.cn |
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| Abstract: |
| Hydrogen sulfide(H2S), a newly found gasotransmitter, has been shown to play crucial roles in the development of plants, as well as in their resistance to various biotic or abiotic stresses. Cadmium is one of the heavy metals that are highly toxic to the growth of plants. In this study, the relationship among the exogenous H2S, the activity of key ATPases, and the expression levels of heavy metal ATPase(HMA) genes, which encode a class of metal transporters, was analyzed under Cd stress conditions, and the mechanism to relieve Cd toxicity in foxtail millet was eventually determined. We determined the contents of soluble sugar and proteins and the activities of ATPases in the foxtail millet seedlings growing under Cd stress conditions. Additionally, the phylogenetic analysis of HMA proteins and the expression level analysis of the HMA genes were carried out under the exogenous application of H2S and Cd. In the seedlings growing under Cd stress, the contents of soluble sugars and proteins were decreased, the activity of ATPases(Ca2+-ATPase, Mg2+-ATPase, and H2+-ATPase) were inhibited, and the expression levels of HMA genes in the P1B-ATPase family were increased. The H2S treatment could increase the content of soluble sugar and the activities of three kinds of ATPases, upregulate the expression levels of HMA1 and HMA2, and down regulate the expression level of HMA3. These results showed that H2S could regulate the activities of P-ATPases to alleviate Cd stress in foxtail millet seedlings. |
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