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Remediation of petroleum-contaminated soils by combined application of biomass materials and nutrients
Received:August 02, 2017  Revised:September 27, 2017
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KeyWord:petroleum hydrocarbons;biochar;nutrients;microorganisms
Author NameAffiliationE-mail
WANG Yan-jie School of Resources and Environmental Science, Hunan Agricultural University, Changsha 410128, China
Institute of Eco-environmental Sciences, Liaoning Shihua University, Fushun 113001, China 
 
LI Fa-yun School of Resources and Environmental Science, Hunan Agricultural University, Changsha 410128, China
Institute of Eco-environmental Sciences, Liaoning Shihua University, Fushun 113001, China 
lnecology@163.com 
RONG Xiang-min School of Resources and Environmental Science, Hunan Agricultural University, Changsha 410128, China  
CHEN Jia-bo Institute of Eco-environmental Sciences, Liaoning Shihua University, Fushun 113001, China  
SHI Li-fang Institute of Eco-environmental Sciences, Liaoning Shihua University, Fushun 113001, China  
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Abstract:
      To study the effects of nutrients, maize straw powder, and biochar on the remediation of petroleum-contaminated soil, four types of remediation treatments were performed in the laboratory. The treatments included unamended soil as the control treatment(T1), soil samples amended with nutrients(nitrogen and phosphorus)(T2), amended with maize straw powder in combination with nutrients(T3), and amended with biochar in combination with nutrients(T4). The remediation experiment was carried out for 90 days. The physicochemical properties of the bioremediated soil were measured after the different treatments. The total bacterial numbers and removal rates of petroleum hydrocarbons during different remediated periods were monitored. The correlation between characteristics of the bioremediated soil and removal rates of petroleum hydrocarbons was analyzed. The results showed that adding maize straw powder and biochar significantly decreased the bulk density of soil, and increased soil porosity and soil field capacity(P<0.05). The total bacterial numbers were increased by adding nutrients and bulk materials, especially in treatment group T4, where the total bacterial numbers reached 7.24×107 CFU·g-1 after 90 days. The removal of total petroleum hydrocarbons and the main hydrocarbon fractions was higher in T3 and T4 after remediation, especially in T4, than in T2 and T1. The physicochemical properties, total bacterial numbers, and removal rate of petroleum hydrocarbons showed significant correlation with each other. Soil porosity and total nitrogen had a great impact on microorganisms, and the effect of microbials on the removal of petroleum hydrocarbons was obvious. Therefore, the addition of biochar and nutrients can improve soil physical properties and provide sufficient nutrients for microorganisms, thereby enhancing the remediation effect of petroleum-contaminated soil.